促凋亡蛋白PDCD5在卵巢上皮性癌中的表达及其与生存关系的研究

Expression of PDCD5 protein in epithelial ovarian cancer and it's relationship with survival

目的探讨促凋亡蛋白PDCD5(Programmed Cell Death 5)在卵巢上皮性癌组织中的表达及其与患者生存时间的关系。方法收集手术切除的卵巢癌组织107例,卵巢良性肿瘤组织16例和正常卵巢组织11例。采用免疫组织化学方法检测各组织内PDCD5蛋白的表达。利用图像分析系统和图像分析软件测定PDCD5蛋白在卵巢上皮性癌、卵巢良性肿瘤及正常卵巢组织中表达的平均光密度值,并结合临床资料进行生存分析。结果①正常卵巢组织,卵巢良性肿瘤组织中PDCD5蛋白呈高表达;卵巢癌组织中PDCD5蛋白呈低表达;图像分析结果显示三组间差异有统计学意义(P<0.05);②PDCD5蛋白在不同FIGO分期和组织学分级的卵巢上皮性癌中表达有显著差异(P<0.05),并且随着FIGO分期和组织学分级的升高,PDCD5蛋白的表达下降;③PDCD5蛋白表达强弱与患者生存时间呈正相关(P<0.05,R=0.731)。结论PDCD5蛋白与卵巢上皮性癌的发生、发展及临床预后有密切关系。

Objective To explore the expression level of Programmed Cell Death 5（PDCD5）protein in epithelial ovarian cancer tissues and to clarify the relationship between PDCD5 and survival time .Methods The expression of PDCD5 protein was analyzed by immunohistochemistry in 107 cases of epithelial ovarian cancer,16 cases of benign ovarian neoplasma and 11 cases of normal ovarian tissues.The image analysis system was used to measure the average optical density.The correlation of PDCD5 protein and patients＇ survival time was analyzed.Results ①PDCD5 protein expression was higher in normal ovarian tissues and benign ovarian neoplasma, but lower in epithelial ovarian cancer tissues.Image analysis demonstrated that the expression of PDCD5 protein among the three groups had significant statistical differences （P0.05）.②The expression of PDCD5 protein in different FIGO stages and histological grades of epithelial ovarian cancer tissues had significant statistical differences（P0.05）, and were downregulated with the increasing of FIGO stages and histological grades.③There was strongly positive correlation between the expression of PDCD5 protein and the survival time （P0.05,R=0.731）.Conclusions The PDCD5 protein has a close relationship with the occurrence, development and clinical prognosis of epithelial ovarian cancer.