重组鸡干扰素-γ对脂多糖引起的肉鸡免疫应激的影响

Effects of Recombinant Chicken Interferon-γ on Lipopolysaccharide Induced Immunological Stress in Broilers

文章旨在研究前期注射重组鸡干扰素-γ(rChIFN-γ)对肉鸡免疫应激的影响。将40羽14日龄肉鸡随机平均分成A、B、C和D 4组。于14、21、28和35日龄给A组和B组肉鸡静脉注射PBS缓冲液1mL.只-1,C组同期静脉注射含rChIFN-γ50μg的PBS缓冲液1mL.只-1,D组同期静脉注射含rChIFN-γ100μg的PBS缓冲液1mL.只-1。于36、38、40日龄,给B、C、D组的肉鸡腹腔注射含有大肠杆菌脂多糖(LPS,按体质量以250μg.kg-1计算)的PBS缓冲液1mL.只-1造模,A组同期腹腔注射PBS缓冲液1mL。记录36日龄至41日龄肉鸡的体质量和采食量,测定第3次注射脂多糖(40日龄)12h肉鸡的血常规和T淋巴细胞刺激指数,并于第3次注射脂多糖后12、24和36h,测定肉鸡血清白细胞介素-1(IL-1)、皮质酮(CORT)的含量。研究表明:(1)在整个免疫应激时期(36～41日龄),A组的平均日增体质量(P<0.01)和平均日采食量(P<0.05)均要高于B、C、D 3组,A组的料重比要显著低于B、C、D 3组(P<0.05);(2)第3次注射脂多糖后12h,C组和D组的白细胞总数显著高于A组和B组(P<0.05);(3)第3次注射脂多糖后12h,C组和D组的淋巴细胞刺激指数显著高于A组和B组(P<0.05);(4)第3次注射脂多糖后12h,A组的CORT含量显著低于B组(P<0.05),第3次注射脂多糖后24h,A组的CORT含量低于B组(P<0.01)、C组(P<0.05)和D组(P<0.05),第3次注射脂多糖后36h,A组显著低于B组(P<0.05);(5)第3次注射脂多糖12h后,A组IL-1显著低于B组(P<0.05),第3次注射脂多糖24h后,A组极显著低于其它各组(P<0.01)。结果显示,前期注射rChIFN-γ,可以缓解免疫应激导致的肉鸡血清中IL-1和皮质酮升高,提高免疫应激时期肉鸡的细胞免疫功能,缓解免疫应激对肉鸡的负面影响。

The objective of this experiment was to research the ettects of injection recombinant chicken interferon-γ （rChIFN-γ） in the prophase on immunological stress broilers. Forty 14-dayold healthy AA broilers were allotted to 4 groups randomly which were group A, group B, group C and group D. The broilers of group A and group B were injected with 1 mL PBS at 14, 21, 28 and 35 days of age. At the same time, the group C was injected with 50 μg rChIFN-γ （in 1 mL PBS） and the group D was injected with 100 μg rChlFN-γ （in 1 mL PBS）. At the time of 36-dayold, 38-day-old and 40-day-old, the broilers of group B, group C, group D were injected with PBS （1 mL） which contained LPS （250 μg · kg^-1 BW）. At the same time, the broilers of group A were injected with PBS （1 mL） only. The performance indices were recorded at the time of 36-day-old. Recorded the weight and intake of each broilers from 36 to 41 days. Hematologic indices and T-lymphocytes proliferation of broilers were assayed at 12 hours post the third injection of LPS. The concentration of interleukin -1 （IL-1） and corticosterone （CORT） in the serum were measured at 12, 24, 36 hours post the third injection of LPS. The results showed that. （1） during the challenge, the growth performance was not affected by rChlFN-γ （P〉0.05）; （2） 12 hours after the third injection of LPS, the white blood cell counts of group C and group D were higher than that of group A （P〈0.05） and group B （P〈0.05）; （3） 12 hours after the third injection of LPS, the T-lymphocyte stimulation index of group C and group D were higher than that of group A and group B （P〈0.05） ; （4） 12 hours after the third injection of LPS, the CORT of group A was lower than that of group B （P〈0.05） ; 24 hours after the third injection of LPS, the CORT of group A was lower than that of group B （P〈0.01）, group C （P〈0.05） and group D （P〈0.05）, 36 hours after the third injection of LPS, the CORT of group A was lower than that of group B （P〈0.05） ; （5） 12 hours after the third injection of LPS, the IL-1 of group A was lower than that of group B （P〈0.05）, 24 hours after the third injection of LPS, the group A was lower than that of the other groups （P〈0. 01）. As the results shown, injection of rChIFN-γ, at prophase could release the IL-1 and CORT in the immunological stress broilers, improve the cell immunity during the stress.