IGF-I与IGFBP-1基因对京海黄鸡生长性状的遗传效应分析

Genetic Effects of IGF-I and IGFBP-1 Genes on the Growth Traits in Jinghai Yellow Chicken

旨在对IGF-I和IGFBP-1基因部分SNPs与鸡生长性状进行关联分析。试验以京海黄鸡为材料,采用PCR-SSCP方法检测2个候选基因的单核苷酸多态性(SNPs),采用一般线性模型(GLM)分析基因型与生长性状的遗传效应。结果表明,IGF-I基因外显子3序列的60bp处有A→G的点突变,在京海黄鸡中检测到AA、AB、BB 3种基因型,A等位基因频率为0.613,B等位基因的频率为0.387;IGFBP-1基因外显子2序列的21bp处有A→T的点突变,104bp处有T→C的突变,在京海黄鸡中检测到CC、CD和DD 3种基因型,C等位基因频率为0.558,D等位基因频率为0.442。IGF-I基因BB基因型个体4周龄体质量显著高于AA和AB型个体(P<0.05);IGFBP-1基因DD基因型个体8周龄体质量显著高于CC基因型个体(P<0.05),4、12和16周龄体质量差异极显著(P<0.01)。因此,推测这些SNPs对京海黄鸡生长性状具有一定的影响,应用于鸡育种过程中的标记辅助选择可以加快育种进程。

The aim of this study was to analyze the association of SNPs in IGF-I and IGFBP-1 genes with chicken growth traits. PCR-SSCP approach was applied to assess the single nucleotide polymorphisms（SNPs） and the general linear model was used to analyze genetic effects between different genotypes and growth traits of the Jinghai Yellow chicken. For the IGF-I gene, three genotypes （AA, AB and BB） were detected in Jinghai Yellow chicken population, the frequency of A and B alleles was 0. 613 and 0. 387, respectively. Sequencing revealed one mutation （G60A） of IGF-I gene in the genotype BB in comparison to the genotype AA. For IGFBP-1 gene, three genotypes （CC, CD and DD） were detected in Jinghai Yellow chicken population, the frequency of C and D alleles was 0. 558 and 0. 442, respectively. Sequencing revealed two mutations （A21T, T104C） of the IGFBP-1 gene in the genotype DD in comparison to the genotype CC.The results showed that individuals with BB genotype of the IGF-I gene had higher bodyweight at 4 weeks compared to individuals with AA and AB genotypes （P〈0.05） ; The individuals with DD genotype of the IGFBP-1 gene had significantly higher bodyweight at 4, 12, 16 weeks compared to the individuals with CC genotype （P〈0.01）. The results suggest that SNPs of IGF-I and IGFBP-1 genes have certain effects on growth traits of Jinghai Yellow chicken, which can be used in marker-assisted selection to accelerate the chicken breeding progress.