摘要
目的:研究12周跑台训练后非酒精性脂肪肝(NAFLD)小鼠肝脏肝细胞癌下调的线粒体转运蛋白(HDMCP)的变化及其对肝脏线粒体功能的影响。方法:雄性8周龄ApoE-/-小鼠48只,随机分为4组,对照组(C,n=12),14周西方膳食组(模型组,M,n=12),继续西方膳食+12周跑台运动组(ME,n=12);西方膳食26周组(MM,n=12)。跑台训练12周,坡度为0°,跑速13m/min,60min/次,每周5次。测定肝脏HDMCPmRNA的表达,线粒体活性氧及H2O2生成,线粒体ATP合成活力,肝脏ATP含量,线粒体态3呼吸、态4呼吸及呼吸控制比(RCR)。结果:耐力训练组(0.53±0.09)肝脏HDMCPmRNA表达显著低于C组(0.72±0.10)、M组(1.24±0.16)、MM组(1.32±0.17);耐力训练组肝脏线粒体ATP合成活力(11.23±2.53)及肝脏组织ATP含量(10.57±2.12)均显著高于MM组(7.36±1.22,8.32±1.41);耐力训练组肝脏线粒体ROS(42.51±4.12)及H2O2生成(18.33±3.10)均显著低于MM组(68.94±5.99,24.31±3.56);耐力训练组线粒体态3呼吸(12.21±1.32)与RCR(2.78±0.89)均显著高于MM组(10.76±1.11,1.75±0.69),而态4呼吸(4.40±1.32)显著低于MM组(6.14±1.45)。结论:12周跑台训练可以显著降低肝脏HDMCP表达,可能进而减少线粒体质子漏,增强线粒体ATP合成,改善线粒体功能。
Objective:To investigate the change of 12-week treadmill training on the HDMCP expression of liver tissue and its effect on mitochondrial function in no-alcholic fatty liver disease(NAFLD)mice.Method:Forty-eight male ApoE-/-mice were divided into four groups randomly:①14-week normal diet group(C group,n=12);②14-week western diet model group(M group,n=12);③western diet plus 12-week treadmill training group(ME group,n=12);④26-week western diet(MM,n=12).Mice were trained on a treadmill for 12 weeks,60 minutes per day,5 days per week,0°grade(at 13m/min).Every period ended,mice were sacrificed and liver was saved for measuring the expression of HDMCP mRNA,and the change of activity of ATP synthetase,and variations of state 3 respiration,state4 respiration and RCR,mitochondrial ROS production and content of ATP in liver tissue.Result:The expression of HDMCP in ME group(0.53±0.09) was significantly lower than that in C group(0.72±0.10),M group(1.24±0.16) and MM group(1.32±0.17).The activity of ATP synthetase(11.23±2.53) and content of ATP in liver tissue(10.57±2.12) in ME group were significantly higher than those in MM group(7.36±1.22,8.32±1.41).The ROS(42.51±4.12) and H2O2(18.33±3.10) production of mitochondria in ME group were significantly lower than those in MM group(68.94±5.99,24.31±3.56).The state3 respiration(12.21±1.32) and RCR(2.78±0.89) in ME group were significantly higher than those in MM group(10.76±1.11,1.75±0.69),however,state4 respiration(4.40±1.32) was lower than that in MM group(6.14±1.45).Conclusion:Twelve training significantly decreases HDMCP expression,which maybe decrease mitochondrial proton leak,enhance the synthesis of ATP of mitochondria,and ameliorate mitochondrial function in NAFLD mice.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2012年第1期12-15,21,共5页
Chinese Journal of Rehabilitation Medicine
关键词
非酒精性脂肪肝
耐力训练
肝细胞癌下调的线粒体转运蛋白
线粒体功能
non-alcoholic fatty liver disease
endurance training
HCC-down-regulated mitochondrial carrier protein
mitochondrial function