雪胆甲素对人非小细胞肺癌A549细胞增殖的抑制作用

Inhibitory effect of cucurbitacinⅡa on proliferation of human non-small cell lung cancer A549 cells

目的探讨雪胆甲素(CucurbitacinⅡa)对人非小细胞肺癌(Non-small cell lung cancer,NSCLC)A549细胞增殖的影响。方法以不同浓度的雪胆甲素作用于A549细胞和小鼠原代培养的脾细胞,采用MTT法检测雪胆甲素对两种细胞增殖的影响;流式细胞术及Annexin V-PE细胞凋亡试剂盒检测雪胆甲素对A549细胞细胞周期及凋亡的影响。结果雪胆甲素对A549细胞和小鼠原代脾细胞的增殖均有抑制作用,且呈浓度依赖性,25～100μmol/L的雪胆甲素对A549细胞的增殖抑制率明显高于脾细胞;雪胆甲素可将大部分A549细胞阻滞于G0/G1和S期,少部分细胞阻于G2和M期,并可促进细胞凋亡。结论雪胆甲素可明显抑制A549细胞增殖,在NSCLC高效低毒的治疗药物的开发中具有重要价值。

Objective To investigate the effect of cucurbitacin Ⅱ a on proliferation of human non-small cell lung cancer （NSCLC） A549 cells. Methods A549 cells and primary murine splenocytes were treated with cucurbitacin Ⅱ a at various concentrations respectively. The effect of cucurbitacin Ⅱ a on proliferation of the two kinds of cells was analyzed by MTF method, while that on cell cycle and apoptosis of A549 cells by flow cytometry and Annexin V-PE cell apoptosis kit. Results Cucurbitacin 1I a showed dose-dependent inhibitory effect on both the proliferations of A549 and primary murine splenocytes. However, the inhibiting rate of cucurbitacin Ⅱ a at concentrations of 25 - 100 μmol / L to A549 cells was significantly higher than that of murine splenocytes. Cucurbitacin Ⅱ a arrested a large portion of cells at Go/GI and S phases, while a small protein at G2 and M phases, and promoted the cell apoptosis. Conclusion Cucurbitacin a Ⅱ inhibited the proliferation of A549 cells significantly, which played an important role in development of high effective and low toxic drugs for NSCLC.