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miRNA调控小鼠涎腺发育基因及相关性研究

Regulation of miRNA to Target Gene and the Relationship between them during Salivary Gland Development in Mice
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摘要 目的:本研究旨在通过小鼠胚胎发育时期涎腺miRNA基因芯片的检测,研究miRNA靶基因调控小鼠涎腺胚胎发育机制以及相互联系。方法:选择在胚胎发育第18天、第19天、及出生当天、出生后3d4个时期形态发育完整下颌下腺作为标本,通过检测基因芯片,建立基因表达谱,运用生物信息学方法,分析这4个时段miRNA表达及相互联系,靶基因的调控关系。结果:建立miRNA与靶基因间调控网络。结论:miRNA在调节小鼠细胞周期、脂质代谢、离子转运、细胞增殖过程中起重要作用,从而使样本在胚胎期到出生后样本发生表型上的变化。 Objective： To study the regulation of miRNAs chips of salivary gland to target gene and the relationship between them during embryonic development period in mice. Method： Submandibular glands of mice were dissected at selected times during development（El8.5, E19.5 .P0 .P3）. Using miRNAchips analysis, miRNA expression pro- files had been established. Then the expression of the miRNA and the relationship between miRNAs and target gene were analyzed by bioinformation method. Results： The miRNA--Gene--Net is established. Conclusion： miRNAs play an important part in regulating cell cycle, lipid metabolic process, ion transport, cell proliferation, and etc. Then, the salivary gland phenotype changes from embryonic stage to birth in mice.
作者 许紫薇 朱形好 方一鸣 张林 任启平
机构地区 温州医学院附属第一医院口腔医疗中心
出处 《口腔医学研究》 CAS CSCD 2012年第1期12-16,共5页 Journal of Oral Science Research
基金 温州市科技局(编号:Y20080090)
关键词 miRNA涎腺基因芯片 生物信息学 miRNA Salivary gland Gene chips Bioinformatics
分类号 R78 [医药卫生—口腔医学]
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参考文献12

  • 1TSUDA N, ISHIYAMA S, LI Y, et al. Synthetic microRNA de--signed to target glioma--associated antigen 1 transcrip- tion factor inhibits division and induces late apoptosis in pan- ereatic tumor cells [J]. Clin Cancer Res, 2006, 12(21) 6557--6564.
  • 2MATHUPALA SP, GUTHIKONDA M, SLOAN AE. RNAi based approaches to the treatment of malignant glioma [J]. Teehnol Cancer Res Treat, 2006, 5(3): 261--269.
  • 3NELSON PT, BALDWIN DA, KLOOSTERMAN WP, et al. RAKE and LNA--ISH reveal microRNA expression and localiza--tion in archival human brain [J]. RNA, 2006, 12 (2) : 187--191.
  • 4Zhang B, Pan X, Cobb G P, Anderson TA. microRNAs as oncogenes and tumor suppressors [J]. Dev Biol, 2007, 302 :1--12.
  • 5Calin GA, Croce CM. MicroRNA--cancer connection: the beginning of a new tale [J]. Cancer Res, 2006, 66 :7390-- 7394.
  • 6Griffiths--Jones S. miRBase: The mieroRNA sequence data- base [J]. MethodsMol Biol, 2006, 342 : 129--138.
  • 7Masters JRW. Epithelial--mesenchymal interactions during lungdevelopment: the effect of mesenchymal mass [J]. Dev Biol, 1976, 51(1) : 98--108.
  • 8Lin H, Hannon GJ. Micrornas: small rnas with a big in gene regulation [J]. Nature Review Genetics, 2004, 5:522--531.
  • 9Lewis BP, Burge CB, Bartel DP. Conserved seed pairing,of- ten flanked by adenosines, indicates that thousands of human genes are microRNA targets [J]. Cell, 2005, 120(1) : 15-- 20.
  • 10谢玉峰,束蓉.miRNA对免疫和炎症反应的调节功能[J].口腔医学研究,2011,27(6):539-541. 被引量：5

二级参考文献33

  • 1Bartel DP. MicroRNAs: Genomics, biogenesis, mechanism, and function [J].Cell, 2004, 116 : 281-297.
  • 2Alvarez--Garcia 1, Miska EA. MicroRNA functions in ani- mal development and human disease [J].Development, 2005, 132 : 4653-4662.
  • 3Lee RC, Feinbaum RL, Ambros V The C. elegans hetero- chronic gene lin--4 encodes small RNAs with antisense com- plementarity to lin--14 [J]. Cell, 1993, 75(5) : 843-854.
  • 4Reinhart BJ, Slack FJ, Basson M, et al The 21-- nucleotide let-- 7 RNA regulates developmental timing in Caenorhabditis elegans [J]. Nature, 2000, 403(6772) : 901-906.
  • 5Doench JG, Sharp PA. Specificity of microRNA target selec- tion in translational repression [J]. Genes Dev, 2004, 18 : 504-511.
  • 6Sonkoly E, Pivarcsi A. Advances in microRNAs: implica- tions for immunity and inflammatory diseases [J].Cell Mol Med, 2009, 13(1) : 24-38.
  • 7Taganov KD , Boldin MP , Chang KJ , et al NF-KB-de- pendent induction of microRNA miR--146 , an inhibitor tar-geted to signaling proteins of innate immune responses [J].Proc Natl Acad Sci USA , 2006 , 103(33) :12481-2486.
  • 8Taganov KD , Boldin MP , Baltimore D. MicroRNAs and Immunity: Tiny Players in a Big Field [J]. Immunity, 2007, 26(2) :133-137.
  • 9Tili E, Michaille JJ, Cimino A, et al Modulation of miR-- 155 and miR--125b levels following lipopolysaceharide/TNF --alpha stimulation and their possible roles in regulating the response to endotoxin shock [J]. Immunol, 2007, 179 5082-5089.
  • 10Wu H, Neilson JR, Kumar P, et al miRNA profiling of na- ive, effector and memory CD8 T cells [J].PLoS ONE, 2007, 2 : el020.

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口腔医学研究
2012年 第1期

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