无蹼壁虎抗肿瘤活性成分对HepG2细胞增殖、迁移及凋亡的影响

Effects of the Gekko Swinhonis anti-neoplasm active component on the proliferation,migration and apoptosis of HepG2 cells

目的观察无蹼壁虎抗肿瘤活性成分(Gekko Swinhonisanti-neoplasm active component,GSAAC)对人肝癌HepG2细胞增殖、迁移及凋亡的影响。方法 GSAAC与体外培养的人肝癌HepG2细胞共培养,分别以MTT法和Transwell小室检测其对细胞增殖及迁移、侵袭的影响;免疫组化法检测PCNA的表达;Hochest33342荧光染色法、TUNEL法观察GSAAC对HepG2细胞的作用;流式细胞术检测细胞周期及早期凋亡率。结果 GSAAC(25～400 mg.L-1)可明显抑制HepG2细胞的增殖、迁移和侵袭能力,呈浓度依赖性;Ho-chest33342、TUNEL染色及流式细胞术结果显示,GSAAC可诱导细胞发生早期凋亡,阻滞HepG2细胞从S期进入G2期。结论 GSAAC可能通过影响细胞周期进而抑制HepG2细胞增殖和迁移并诱导细胞发生凋亡,进而实现抑制肿瘤的作用。

Aim To investigate the effects of the Gekko Swinhonis anti-neoplasm active component（GSAAC） on the proliferation,migration and apoptosis of HepG2 cells.Methods HepG2 cells were treated with GSAAC.Cell proliferation and migration were determined by MTT and Transwell assay after treatment.The expression of PCNA was detected by immunohistochemistry.Hochest33342 fluorescence staining and TUNEL staining were used to observe the morphological changes of HepG2 cells.Early apoptosis and cell cycle distribution were assessed by flow cytometry.Results GSAAC significantly inhibited the proliferation,migration and induced the early apoptosis of HepG2 cells;GSAAC blocked HepG2 cells to go to G2 phase from S phase.Conclusion The intrinsic mechanism of GSAAC might relate to the blockage of HepG2 cells to go to G2 phase from S phase thus to inhibit the proliferation,migration and induce the early apoptosis of HepG2 cells.