机械伤促进红色砂梨着色的机制分析

The Mechanism Analysis of Anthocyanin Accumulation in Red Chinese Sand Pears Enhanced by Wounded Treatments

以红色砂梨(Pyrus pyrifolia Nakai)品种'满天红'为试材,研究了采后机械伤处理对果实着色相关的生理指标和基因表达的影响。结果表明:机械伤处理能够有效促进果实受伤部位花色苷的积累,并伴随着该部位果实硬度显著下降,乙烯释放速率显著上升,花色苷合成相关基因PpF3H、PpANS、PpUFGT和PyMYB10表达量显著上调,而对照果实仅发生了轻微着色,且未检测到乙烯的释放。据此推测乙烯可能参与了红色砂梨果实花色苷合成的调控,对花色苷合成具有促进作用。机械伤处理可以作为一种有效的研究方法用于果实着色调控机制的研究。

Effects of wounded treatments on postharvest coloration and related physiology and gene expression in red Chinese sand pear（Pyrus pyrifolia Nakai ） ＇Mantianhong＇ were studied. Results showed that wounded treatments decreased fruit firmness, increased ethylene production rate of fruit, enhanced postharvest anthocyanin accumulation in injured part of red Chinese sand pear, and upregulated the transcript level of anthocyanin biosynthetic related genes： PpF3H, PpANS, PpUFGT and PyMYB10, markedly. Ethylene production of control fruit was under the detection limit, and only a trace amount of anthocyanin was detected in control fruit. Results above indicated that ethylene might be involved in the regulation of anthocyanin accumulation in red Chinese sand pear, and improve the level of anthocyanin accumulation in red Chinese sand pear. It was suggested that wounded treatments could be used as a mean to better understand the regulation mechanism of anthocyanin biosynthesis in fruits.