酚类物质对东北山樱生长及呼吸关键酶的影响

Effects of Phenolic Compounds on the Growth and Key Enzymes in Respiration of Cerasus sachalinensis

以东北山樱(Cerasus sachalinensis Kom.)幼苗为试验材料,研究了根施酚类物质对羟基苯甲酸和香豆素对其生长和呼吸关键酶的影响。结果表明,低浓度的香豆素显著促进东北山樱幼苗生长,提高幼苗光系统Ⅱ(PSⅡ)最大光化学效率、呼吸底物含量、中间产物丙酮酸含量和苹果酸脱氢酶(MDH)活性,不影响糖酵解途径(EMP)和磷酸戊糖途径(PPP)关键酶活性;高浓度香豆素抑制生长,降低PSⅡ最大光化学效率、淀粉含量、异柠檬酸脱氢酶(IDH)和磷酸果糖激酶(PFK)活性。对羟基苯甲酸及其与香豆素的混合溶液在3个作用浓度(0.1、1.0和10 mmol·L-1)下均不同程度地抑制幼苗生长,降低柠檬酸含量,促进PPP关键酶活性提高。低浓度对羟基苯甲酸对幼苗PSⅡ最大光化学效率和呼吸底物含量无影响,但提高丙酮酸含量、己糖激酶(HK)、丙酮酸激酶(PK)和琥珀酸脱氢酶(SDH)活性,降低IDH、MDH活性;高浓度对羟基苯甲酸使幼苗PSⅡ最大光化学效率、呼吸底物和中间产物含量、PFK、PK、IDH、MDH活性下降,HK活性提高。

Effects of two phenolic compounds （phydroxybenzoic acid and coumarin） on growtia ana pivotal respiratory indicators were studied using Cerasus sachalinensis Kom. seedlings as materials. The results showed that the growth of C. sachalinensis seedlings was highly promoted by low concentration of coumarin, and which also enhanced the PS II maximum photochemical efficiency, respiratory substrate content, pyruvate content of metabolic intermediates and MDH activity. However, the key enzyme activities of EMP and PPP were not affected. Coumarin at high concentrations showed inhibitions to seedlings, while brought the PS II maximum photochemical efficiency, starch contents, IDH activities and PFK activities down. phydroxybenzoic acid and the mixed solution of the two compounds at the 3 testing concentrations （0.1, 1.0 and 10 mmolL＂1 ） both restrained seedling growth at certain extent. Contents of citric acid were reduced and key enzyme activities of PPP were improved. The PS II maximum photochemical efficiency andrespiratory substrate content were not changed by low concentration of phydroxybenzoic acid. However, pyruvate content, HK, PK, SDH activities were promoted. On the contrary, IDH, MDH activities were lowered, phydroxybenzoic acid at high concentrations showed suppressions on PS II maximum photochemical efficiency, respiratory substrate contents, respiratory intermediates contents, and PFK, PK, IDH, MDH activities, but enhancement on HK activity.