新型筛选标记磷酸甘露糖异构酶基因在转基因植物中的应用

Applications of Phosphomannose Isomerase-based Selection System in Transgenic Plants

广泛应用于植物遗传转化的抗生素和除草剂选择标记对环境生态和人类健康可能具有潜在风险,因此生物安全筛选标记已成为当前植物转基因研究的热点。磷酸甘露糖异构酶基因(PMI)是一种新型生物安全选择标记基因,全面介绍了其作用原理、生物安全性、在植物转化中的应用与检测等,深入分析了其在植物转化中作为筛选标记存在的问题与不足,并就其发展前景进行了展望,以期为在转基因植物中的广泛应用奠定基础。

Selective marker gene encoding antibiotic and herbicide, widely used in plant transformation, have potential risk for environment and human health. Therefore, non-resistance marker genes with bio-safety become a research hotspot in the area of transgenie plants. This paper presented a novel selective system for plant transforma- tion, which utilizes the pmi gene encoding phosphomannose-isomerase as selective marker gene, and summarized the progresses of PMI on its function principle, bio-safety evaluation, application and detection in plant transformation. The paper also analyzed the existing problems of PMI as selective marker gene, and prospected the development trends of PMI, so as to establish a solid foundation for its extensive utilization in transgenic plants.