针刺提插补泻足三里对血虚证模型家兔RBC,Hb,MCV的影响

Influences of lifting-thrusting reinforcing-reducing method in Zusanli (ST36) on RBC, Hb and MCV in rabbit model of blood-deficiency syndrome

目的通过观察针刺提插补泻"足三里"穴对血虚证家兔红细胞计数(RBC)、血红蛋白(Hb)、红细胞体积(MCV)的影响,探讨提插补泻与补泻效应的关系。方法将60只家兔随机分为5组:A组(空白组)、B组(模型对照组)、C组(留针组)、D组(提插补法组)、E组(提插泻法组)。隔天治疗1次,共计10次。结果针刺处理后,B,C,D,E组家兔外周血RBC和Hb均明显升高,分别与针刺处理前比较,均有显著差异(P<0.05)。对各组家兔的RBC和Hb在针刺处理结束后的第2天、第17天、第32天这3个时间点进行组间比较,均无统计学差异(P>0.05)。针刺处理结束后第17天,B,C,D,E组家兔外周血中MVC与针刺处理前比较,有显著差异(P<0.05)。结论重插轻提的提插补法与重提轻插的提插泻法在虚证的病理状态下无明显差异,未显示出补泻效应。

Objective To discuss the relationship between lifting-thrusting reinforcing-reducing method and reinforcing-reducing effect through observing the influences of lifting-thrusting reinforcing-reducing method in Zusanli （ST36） on red blood count （RBC）, hemoglobin （Hb） and mean corpuscular volume （MCV） in rabbit model of blood-deficiency syndrome. Methods All rabbits （n=60） were randomly divided into five groups including A group （blank group）, B group （model control group）, C group （retaining needle group）, D group （lifting-thrusting reinforcing method） and E group （lifting-thrusting reducing method）. All groups were treated one time every other day for 10 times. Results After needling, the levels of RBC and Hb in peripheral blood increased significantly in B, C, D and E groups compared with those before needling （P0.05）. The comparison in the levels of RBC and Hb among all groups showed that the difference was not statistically significant （P0.05） on the 2nd, 17th and 32nd days after needling. On the 17th day, MCV in peripheral blood was significantly different in B, C, D and E groups compared with that before needling （P0.05）. Conclusion There was no significant difference between lifting-thrusting reinforcing method （thrusting needle with force and lifting it gently） and lifting-thrusting reducing method （lifting needle with force and thrusting it gently） under the pathological condition of deficiency syndrome and no reinforcing-reducing effect showing.