HBeAg阳性母亲流产胎儿肝及肝外脏器HBV标志的检测

DETECTION OF HBV MARKERS IN LIVER AND EXTRAHEPATIC ORGANS OF ABORTED FETUSES BORN TO HBEAG POSITIVE MOTHERS

应用免疫金银染色法和原位核酸杂交技术同步检测9例HBsAg和HBeAg均阳性母亲流产胎儿的肝、胸腺、脾、胰中的HBsAg、HBcAg和HBV-DNA.结果,在肝(5例)、胸腺(1例)、脾(3例)、胰(2例)中测出HBsAg及HBV-DNA.其中2例胎肝细胞并呈HBcAg阳性,但在胸腺、脾、胰中未检出HBcAg.HBsAg分布主要为胞浆型,也见有膜型;HBV-DNA及HBcAg主要分布于胞浆内,偶见于核内.此种分布特点,提示HBV处于复制相.在肝脏中,HBsAg、HBcAg及HBV-DNA除见于胞浆外,还见于造血灶中造血细胞内.上述结果表明,HBV已感染胎儿肝脏、胸腺、脾脏、胰腺以及肝内造血细胞.

Using immune gold silver stain (IGSS) and in-situ hybridization techniques, the author made an assay simultaneously on the HBV antigens and HBV-DNA in the livers, thymuses, spleens and pancreases of 9 aborted fetuses born to HBsAg-HBeAg positive mothers. The result showed that HbsAg and HBV-DNA were positive in hepatocytes of 5, in thymus of 1, in spleens of 3 and in pancreases of 2 fetuses. HBcAg was positive only in hepatocytes of 2 fetuses and negative in all fetal thymus, spleen and pancreas. HBsAg was present mainly in the cytoplasm or cell membrane. HBVDNA and HBcAg were mainly seen in the cytoplasm and occasionally in nucleus. In addition, HBsAg, HBcAg and HBV-DNA could also be found in liver hemopoietic cells. The above mentioned results suggest that the hepatocytes, thymocytes, splenocytes, pancreatic cells as well as the liver hemopoietic cells of the fetuses had been infected by HBV before their being born to HBeAg positive mothers.