Caspase-3在慢性乙型肝炎中的表达及意义

Expression and significance of Caspase-3 in chronic hepatitis B

目的通过对照观察正常人与慢性乙型肝炎患者肝组织Caspase-3及血清谷丙转氨酶水平,探讨Caspase-3在慢性乙型肝炎发病中的意义。方法应用免疫组化方法对66例CHB患者和15例正常肝组织进行Caspase-3检测,并检测CHB患者血清谷丙转氨酶水平。结果乙型肝炎患者Caspase-3表达显著高于正常肝组织,差异有统计学意义(P<0.05)。Caspase-3表达与CHB炎症分级显著正相关(P<0.05),与血清ALT和CHB纤维化程度差异无统计学意义(P>0.05)。结论 Caspase-3表达的活性随肝炎炎症程度增高而增强,从而对慢性乙型肝炎患者肝细胞凋亡产生影响作用。

Objective To explore the role of Caspase-3 in chronic hepatitis B and their clinical significance by the ways of Control-studying to observation the expression of Caspase-3 and detecting alanine aminotransferase expression level in chronic hepatitis B and normal hepatic tissue.Method Adopting immunohistochemical methods to test the expression of Caspase-3 in liver biopsy specimens which obtained from 66 adults CHB and 15 normal liver tissues and detect alanine aminotransferase（ALT） expression too.Results The expression of Caspase-3 in hepatocytes of CHB patients was significantly higher than those in normal liver（P0.05）.Caspase-3 in hepatocytes of CHB patients was positively related to the grade of inflammation in CHB（P0.05）,but it did not relate to the stage of fibration（P0.05） and ALT activity（P0.05）.Conclusions Caspase-3 expression in hepatocytes of CHB patients grows higher with development of inflammation in CHB,thus impacts on the apoptosis of liver cell in chronic hepatitis B patients.