重度心肌挫伤心肌细胞中COX-2表达的实验研究

Study on COX-2 expression in cardiac muscle cell of severe myocardial contusion

目的观察大鼠重度心肌挫伤后不同时间段挫伤区周围心肌细胞的COX-2阳性染色强度变化与损伤后经过时间之间的关系。方法运用免疫组织化学技术(SABC法),结合计算机彩色图像分析技术观察大鼠实验性重度心肌挫伤后COX-2的染色变化。结果 COX-2参与了重度心肌挫伤后细胞的损伤.结论心肌挫伤后的时序性变化规律使COX-2有可能成为重度心肌挫伤时间推断的客观指标以及生前伤和死后伤的鉴别指标。

Objective To observe the COX-2 of cardiac muscle cells round the wound area in different time of severe myocardial contusion,expore the relationship between COX-2 positive staining change and injury time.Method Combining immunohistochemistry method and computer color image analytical technique to observation the expressions of COX-2 after severe myocardial contusion.Results COX-2 may play a role in severe myocardial contusion.Conclusions Basis on the time sequence regularity,COX-2 may play an objective indication of time ratiocination after severe myocardial contusion,it was also the identification index of judgment the injuries before or after dead.