蘑菇罐头中苯甲酸的浊点萃取-高效液相色谱测定法

Determination of Benzoic Acid in Canned Mushroom by Cloud Point Extraction-HPLC

目的建立浊点萃取(CPE)对蘑菇罐头中的苯甲酸进行萃取富集、高效液相色谱-紫外检测器进行检测分析的方法。方法采用Tween-20为萃取剂,CPE方法的优化条件:Tween-20的浓度为2%(体积分数)、(NH4)2SO4浓度为300 g/L,平衡温度为90℃,平衡时间为10 min。结果在上述实验条件下,蘑菇罐头样品的加标回收率为92.0%～102.0%,RSD为1.50%。结论该方法简便、快速、灵活、污染少,实际应用性好。

[Objective]To establish the method for extraction and concentration of benzoic acid from canned mushroom by cloud point extraction（CPE）,determination of it by high performance liquid chromatography analysis. [Methods]Tween-20 was chosen as extraction solvent.The optimum CPE conditions were 2%（V/V） Tween-20,300 g/L（NH4）2SO4,equilibration temperature 90 ℃,equilibration time 10 min. [Results]Under this experimental conditions,the recoveries were 92.0%~102.0%,and RSDwas 1.50%. [Conclusion]This method is simple,fast,flexible,with less pollution and well practical utility.