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多重等位基因特异性PCR-熔解曲线分析快速定型β珠蛋白基因突变 被引量：2

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摘要 目的建立自动化、高通量、准确快速检测β珠蛋白基因突变的技术。方法在反应体系中加入新型荧光染料LC Green进行实时荧光聚合酶链反应,反应结束后,以0.1℃/s变化速度从60℃至95℃每隔3 s记录一次荧光值,获得熔解曲线,根据得到的熔解曲线和Tm值分析定型β地中海贫血的CD41-42、IVS 2nt654、CD17、CD71-72四种等位基因突变。结果根据Tm值能准确判断基因型,检测CD41-42、IVS 2nt654、CD17、CD71-72四种等位基因的Tm值分别为78±1℃、80±1℃、76±1℃、79±1℃。结论该技术具有自动化程度高、不需荧光标记探针、成本低、易质控、防污染、高通量等优点,适于临床推广应用。
作者 张银汉 李建新 喻晶 刘淼 刘晓翌 吴夏枫
机构地区 北京大学深圳医院检验科 北京大学深圳医院妇产科
出处 《中国优生与遗传杂志》 2011年第12期17-18,共2页 Chinese Journal of Birth Health & Heredity
基金 深圳市科技局立项(项目编号:201003055)
关键词 LC Green 熔解曲线分析 Β地中海贫血 珠蛋白 基因突变
分类号 R556.61 [医药卫生—血液循环系统疾病]
  • 相关文献

参考文献10

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二级参考文献15

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共引文献132

同被引文献27

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引证文献2

二级引证文献10

中国优生与遗传杂志
2011年 第12期

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