高效液相色谱法测定尿中马尿酸的规范化研究

Standardized experimental study on the determination of hippuric acid in urine by HPLC

目的:进一步完善和规范化甲苯接触者尿中马尿酸的高效液相色谱测定法。方法:采用ODS C18柱,流动相为甲醇-0.01 mol/L磷酸(40+60),流量为1.0 ml/min,检测波长228 nm。结果:马尿酸在10μg/ml～500μg/ml范围内线性良好,相关系数r为0.9999,平均加标回收率为99%,平均相对标准偏差RSD为1.1%,最低检测浓度0.41 mg/L。结论:该方法快速、准确、灵敏度高,有良好的重现性和稳定性,可用于职业接触甲苯人群尿中马尿酸的测定。

Objective：To explore a further research on the analytical method for the determination of hippuric acid in urine by HPLC.Methods： C18 column was used to determine the hippuric acid.The mobile phase was used with Methanol-0.01 mol/L phosphoric acid（40∶ 60） at 1.0 ml/min.The detection wavelength was at 228 nm.Results： Calibration curves of hippuric acid were linear in the range of 10 μg/ml^500 μg/ml,with a correlation coefficient more than 0.9999.The average recovery was 99% and the average RSD was 1.1%.The minimum detectable concentration was 0.41 mg/L.Conclusion： The method is convenient and accurate with good reproducibility and stability,and can be used to determine hippuric acid in urine of toluene exposure workers.