荧光定量PCR技术检测肠道志贺菌的临床应用

Clinical application of real-time PCR in determination of intestinal Shigella

目的:比较荧光定量PCR技术和传统培养方法检测肠道志贺菌的效果。方法:利用中山大学达安基因股份有限公司研制开发的志贺菌核酸检测试剂盒,检测我院诊治的腹泻病人332份大便中的志贺菌核酸,并以传统的沙门志贺菌(SS)培养基进行细菌培养的结果作为对比。结果:332份腹泻病人大便标本,用培养方法检测,志贺菌阳性59例;用志贺菌核酸试剂盒检测有107例阳性。荧光定量PCR检测方法与培养方法对比,敏感度为100.00%,特异性为82.43%。结论:荧光定量PCR技术敏感度高、特异性强、适合于临床大标本量的检测。

Objective：To compare the effect between real-time PCR technique and conventional culture assay for the quantitative detection of intestinal bacteria.Methods： 332 stool samples from diarrhea petients in our hospital were detected quantitatively by real-time PCR kits of Shigella nucleic acid produced by Da an gene company.Furthermore,the results were compared with that of conventional Salmonella and Shigella（SS） culture assay.Results： 59 strains of Shigella were isolated by culture and 107 positive Shigella were isolated by real-time PCR from 332 stool samples of diarrhea patients.By comparison,the sensitivity was 100.00% and specificity was 82.43% by real-time PCR.Conclusion： The real-time PCR technique is a method with high sensitivity and specificity,which is more suitable to detect the Shigella from clinical samples.