电针肝俞、期门对肝气郁结模型大鼠行为学及HPA轴相关激素的影响

Effect of Electroacupuncture at Gan Shu,Qi Men on Changes of Behaviors and Related Hormones of Hypothalamus-Pituitary-Adrenal Axis in Rats with Syndrome of Stagnation of Liver-Qi

目的:探讨电针对肝气郁结模型大鼠行为学影响及下丘脑-垂体-肾上腺(HPA)轴相关激素的调节作用。方法:将Wistar大鼠30只随机分为正常对照组、肝郁模型组、电针治疗组,通过开野实验(Open-Field Test)及蔗糖水消耗实验进行大鼠行为学检测,运用ELISA法检测大鼠血清促肾上腺皮质激素释放激素(CRH)、皮质酮(CORT)含量,3组之间进行比较。结果:与正常组相比,模型组Open-Field Test水平运动得分和垂直运动得分明显减少,蔗糖水偏嗜度明显减少,血清CORT、CRH的含量明显升高,而电针明显增加模型大鼠的水平得分、垂直得分及蔗糖水偏嗜度,并可阻抑血清CRH、CORT的过度分泌。结论:肝气郁结大鼠存在活动度降低、探究行为减少、快感缺失和HPA轴亢进,电针肝俞、期门可抑制CRH、CORT的过度分泌,缓解HPA轴亢进,外在体现为对模型大鼠行为学的改善。

Objective：To investigate the effect of electroacupuncture（EA） on changes of behaviors and some related hormones of the hypothalamus-pituitary-adrenal（HPA） axis in rats with stagnation of liver-qi syndrome. Methods：The rats were randomly divided into three groups： normal group,model group,EA group,n=10.We judged the behavioral changes of model rats and the behavioral action of EA in treating the stagnation of liver-qi syndrome through Open-Field Test and partial addicted to sugar water test.The content of adrenocorticotropic hormone-releasing hormone（CRH） and serum corticosterone（CORT） were measured by ELISA method. Results：The horizontal movement scores and vertical movement scores of rats in model group both significantly reduced compared with the normal group（P0.01）,as well as the degree of partial addicted to sugar water of model group rats decreased significantly（P0.01）.The content of CRH and CORT in serum of model group rats were significantly higher（P0.01）.The horizontal movement scores,vertical movement scores and the degree of partial addicted to sugar water of EA group increased compared with the model group,as well as CRH and CORT contents lowered significantly in EA group. Conclusion：Rats with stagnation of liver-qi syndrome have behavioral changes including lack of pleasure,decreasing of exploration activities and hyperactivity of HPA axis,whereas electroacupuncture can promote recovery of the rats＇ abnormal behaviors and inhibit the hyperactivity of the HPA axis by adjusting the secretion of CRH and CORT.