针刺对更年期脑缺血大鼠GFAP和OX42表达的影响

The Effect of Acupuncture on GFAP and OX42 Expression of Rats with Climacteric Cerebral Ischemia

目的:研究针刺对更年期脑缺血大鼠缺血区脑组织GFAP和OX42蛋白表达的影响,探讨针刺对神经损伤的保护作用及可能机制。方法:雌性SD大鼠,经筛选为自然更年期,随机分为假手术组、缺血模型组和针刺组,采用右侧大脑中动脉阻塞(MCAO)法建立脑缺血动物模型。针刺治疗后,采用Ber-derson神经体征评分法,观察大鼠神经行为学变化,光镜观察脑组织的病理学变化;免疫组织化学法检测脑组织中GFAP和OX42蛋白表达。结果:针刺能明显改善大鼠脑缺血后24 h和7 d的神经损伤症状;改善脑组织病理形态学损伤状态;对缺血24 h和7 d脑组织中GFAP和OX42蛋白表达有不同程度降低,与模型组相应时间点相比较,差异有统计学意义(P﹤0.05或P﹤0.01)。结论:针刺能够抑制胶质细胞GFAP和OX42的活化,减轻脑缺血后的炎症级联反应,从而改善脑组织病理学损伤状态,起到神经保护作用。

Objective：To research the influence of GFAP and OX42 protein expression of MCAO climacteric female rats by electro-acupuncture,and investigate the protective effect of neurological damage and possible mechanisms. Methods：Healthy naturally climacteric female SD rats,use intraluminal MCAO rat model,after the electroacupuncture treatment,Experiments were carried out after the modeling 24 h and 7 d.Neurological signs by Bederson neurological score behavioral changes were observed;Use light microscope morphological changes in brain tissue;It was detected by immunohistochemistry in brain tissue GFAP,OX42 expression. Results：Electroacupuncture can be significantly improved after cerebral ischemia in rats 7 d or 24 h symptoms of nerve injury;It could improve the pathological brain damage state and reduce varying degrees of the protein expression of GFAP and OX42 in 7 d and 24 h ischemic brain tissue.Compared with model group,there were significant differences（P﹤0.05 or P﹤0.01）. Conclusion：The electroacupuncture can significantly improve the state of brain tissue pathological damage,and improve after MCAO in rats 7 d or 24 h and symptoms of neurological damage.Electroacupuncture can be through activation Inhibition of glial cells the GFAP and OX42,24 h and 7 d after ischemia,and inhibit the post-ischemic 24 h and 7 d inflammatory cascade.Reduce the blood-brain barrier damage so as to improve brain histopathological damage.It plays a positive role in brain protection.