分散液液微萃取-气相色谱法测定塑料浸泡液中17种邻苯二甲酸酯

GC Determination of 17 Phthalates in Simulated Sweat Soaked with Plastics by Dispersive Liquid-Liquid Microextraction

将已粉碎的塑料样品用模拟汗液在37℃下振荡1h。向提取液中加入由丙酮(作分散剂)和四氯化碳(作萃取剂)充分混和的混合液,以3 000r.min-1转速离心5min,分取1μL有机相进样作气相色谱分析。色谱分离时采用HP-5毛细管柱,采用氢火焰离子化检测器进行测定。提取液经上述分散液液微萃取分离富集后测定其中17种邻苯二甲酸酯类化合物(PAE′s)。在所试验的5种塑料制品样品的提取液中均检测出邻苯二甲酸二(2-乙基己基)酯,其最大溶出量为51.47μg.L-1。在塑料包装袋的提取液中检出了邻苯二甲酸二(2-乙氧基乙基)酯,其最大溶出量为111.14μg.L-1。17种PAE′s的检出限(3S/N)在0.15～5.05μg.L-1之间。

Smashed plastics sample was shaked with simulated sweat at 37 ℃ for 1 h.A mixture of acetone（as dispersion agent） and CCl4（as extractant） was added promptly to the extract,mixed thoroughly and centrifuged at 3 000 r·min-1 for 5 min.One μL of the organic phase containing the phthalates（PAE′s） was taken for GC analysis,using HP-5 capillary column for separation and hydrogen flame ionization detection for determination.Amounts of 17 PAE′s in the simulated sweat as dissolved from the plastics,was determined.Di-2-ethylhexyl phathalate was detected in extracts of 5 plastics,with 51.47 μg·L-1 as the maximum dissolved amount.Bis-2-ethoxyethyl phthalate,with the maximum amount of 111.14 μg·L-1,was found in extracts of packing bags.Detection limits（3S/N） of the 17 PAE′s were obtained in the range of 0.15-5.05 μg·L-1.