摘要
fw2.2基因是影响番茄果重的一个重要数量性状基因,在心皮的细胞分裂中起负调控作用。本研究以番茄fw2.2基因的序列为探针,从花生EST数据库中筛选同源序列。根据花生的EST拼接序列设计引物对花生进行扩增,目标产物测序后,将推测的氨基酸序列与其他植物fw2.2基因编码的氨基酸序列进行比对,同源性为34.78%～66.85%。半定量RT-PCR结果表明,该基因在野生种和栽培种中的表达存在差异。将fw2.2基因连接到植物表达载体,通过农杆菌介导法转化花生品种花育23号,获得了12个PCR阳性的独立转化子。
fw2.2 gene is an important quantative trait locus（QTL） affecting fruit weight in tomato,and can reduce cell division in carpels as a negative regulator.In this experiment,fw2.2 gene in tomato was prepared as probe to screen homologous sequence from peanut EST database.Then,according to the joint EST sequence,a pair of primer was designed to amplified the fw2.2 gene of peanut.Compared with other plants,the similarity range of amino acid sequence was between 34.78% and 66.85%.Expression difference was detected between wild and cultivated materials by semi-quantitative RT-PCR.After transformation mediated by Agrobacterium tumefaciens,12 independent transformants of peanut cultivar Huayu 23 were identificated by PCR amplification.
出处
《核农学报》
CAS
CSCD
北大核心
2011年第6期1123-1128,1210,共7页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金(30871544)
山东省自然基金青年基金(ZR2011CQ026)
山东省中青年科学家奖励基金(BS2009NY028)
青岛农业大学博士启动基金(630716)
