摘要
A new method was developed for simultaneous determination of levels of AMP,ADP and ATP in blood by anion-exchange chromatography.The three adenosine phosphates were separated by Dionex IonPac AG18（50 mm×4 mm） guard column and IonPac AS 18（250 mm×4 mm） analytical column using a gradient method and detected with a suppressed conductivity detector. The detection limits（S/N=3） of AMP,ADP and ATP were 38,47,108 ng/L,respectively.The relative standard deviations of retention time,peak area and peak height were all less than 1.87%and a good linear relationship was obtained.This method was applied to analyze human blood samples.
A new method was developed for simultaneous determination of levels of AMP,ADP and ATP in blood by anion-exchange chromatography.The three adenosine phosphates were separated by Dionex IonPac AG18（50 mm×4 mm） guard column and IonPac AS 18（250 mm×4 mm） analytical column using a gradient method and detected with a suppressed conductivity detector. The detection limits（S/N=3） of AMP,ADP and ATP were 38,47,108 ng/L,respectively.The relative standard deviations of retention time,peak area and peak height were all less than 1.87%and a good linear relationship was obtained.This method was applied to analyze human blood samples.
基金
financially supported by National Natural Science Foundation of China(Nos.20775070 and J0830413)
Zhejiang Provincial Natural Science Foundation of China(Nos.R4080124 and Y4090268)
