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狂犬病毒糖蛋白衍生物作为靶脑载体初探 被引量：3

New Brain Delivery Vector: Rabies Virus Glycoprotein-derived Peptide
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摘要 血脑屏障(blood-brain barrier,BBB)阻碍了具有治疗潜力的大分子化合物从外周组织进入脑内。为了寻找一种高效、快速通过BBB的靶向性载体,实验通过罗丹明B标记的狂犬病毒糖蛋白衍生肽(RDP)注射入昆明小鼠体内,并于15 min、5 h取大脑、脊髓及肝、肾等外周组织,冷冻切片观察其在体内的分布,并通过构建pET28a-RDP-luciferase重组质粒,结果发现融合蛋白能快速地穿过血脑屏障分布于中枢神经系统,为治疗中枢神经系统的药物开发提供新的思路。 Blood-brain barrier（BBB） prevents the transport pathway of potential macromolecular protein from blood to brain in order to find an efficient brain delivery vector,the rhodamine B labeled rabies virus glycoprotein-derived peptide（RDP）is applied to inject Kunming mice,and 15 min or 5 h take the brain,spinal cord,liver,kidney and so on.Frozen sections were observed the distribution.The RDP gene and luciferase gene were fused to the pET28a plasmid.The fusion protein can cross the BBB and distributed in the central nervous system rapidly.RDP can be used in future engineering of therapeutic fusion proteins for BBB drug delivery.
作者 王逸麟 付爱玲
机构地区 西南大学药学院
出处 《科学技术与工程》 2011年第36期8974-8977,共4页 Science Technology and Engineering
关键词 狂犬病毒糖蛋白衍生物 血脑屏障 靶脑转运 rabies virus glycoprotein-derived peptide blood-brain barrier brain-targeted carrier
分类号 R962 [医药卫生—药理学]
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参考文献4

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共引文献3

同被引文献69

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引证文献3

二级引证文献6

科学技术与工程
2011年 第36期

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