蟾蜍毒素对H22荷瘤小鼠肝癌细胞凋亡的影响

Effect of Ethanol Extract of Toad Venom on the Inducing Apoptosis in Bearing-H22 Tumors Mice

[目的]探讨醇溶性蟾蜍毒素对H22荷瘤小鼠肝癌细胞凋亡的影响。[方法]用乙醇溶解蟾蜍分泌物原浆,采用减压蒸馏等方法进一步部分分离纯化,获得醇溶性的蟾蜍毒素混合物(EET),将其作用于H22荷瘤小鼠模型,计算肿瘤抑制率,应用光、电镜法观察H22肝癌细胞凋亡的变化。[结果]蟾蜍毒素组和氟脲嘧啶组的肿瘤抑制率分别是25.6%和30.8%(P<0.05),与对照组比较,肿瘤团块较小,细胞体积较小,异型性不明显,病理性核分裂较少,间质血管较少,坏死现象较轻。电镜下可见凋亡小体形成。[结论]蟾蜍毒素可通过诱导H22荷瘤小鼠肝癌细胞凋亡来达到抗肿瘤的作用。

[Objective]To observe the effect of Ethanol Extract of Toad Venom on the a inducing apoptosis in bearing-H22 tumors mice.[Methods]The models of bearing solid tumors were made by incubated H22 cell suspension to back.All 60 mouse were distributed into 3 groups and injected 0.5mg/kg Ethanol Extract of Toad Venom or Fluorouracil to abdominal cavity,respectively.The mouse of normal control group were injected normal sodium to abdominal cavity.The inhibition rate of solid tumors in mice were calculated.Using the method of light microscope（LM） and electron microscope（EM） to observe the morphology.[Results]The inhibition rate of tumor were25.6% and 30.8%（P0.05） administrated 0.5mg/kg Ethanol Extract of Toad Venom or Fluorouracil respectively.To be compared with the control group,the ethanol extract of toad venom group has following characteristics： the tumor ball and the cell volum are smaller,the atypia is not evident,and the pathological karyokinesis and interstitial blood vessel are fewer.It has necrosis.Through the observation of EM,we found the formation of apoptotic body.[Conclusion]The results suggest that Ethanol Extract of Toad Venom can inhibit tumor cells＇ growth by inducing apoptosis.