不同发育时期甜菜花粉TTC染色活力的测定

Detection of pollen TTC stainability for sugar beet in different developing phase

花粉活力直接影响作物授粉、受精及种子结实率，为了解甜菜品种（系）的花粉生活力及特性，给甜菜引种栽培和杂交育种工作创造有利条件，本试验以自育2倍体甜菜授粉系为试验材料，采用TTC染色方法检测花粉活力，研究了甜菜花粉在不同发育时期的染色活力，确定了甜菜花粉染色活力的快速检测方法。试验结果显示：处于不同发育及散粉期甜菜花粉的染色活力具有显著差异，未开裂花药内花粉粒染色活力百分率30％～55％，花药刚开裂时，花粉染色活力达90％以上，散粉后的散落花粉染色活力最低，甜菜花粉于阳光下直接照射1h后，花粉迅速丧失染色活力。

Pollen viability affects crops pollination, fertilization and seeding ratio. To understand pollen viabilityand character of sugar beet lines, and bring up advantage for crossbreed work, the staining viability of sugar beet pollen in different developing phase was studied using TI＇C dyeing method. Detection method of sugar beet pollen stainbility was fixed on. The result showed that the rate of pollen stainability of sugar beet pollen among different growth period was significant difference. The rate of pollen stainability in anther was between 30% and 55% ; the rate of pollen stainability when anther just craze can up to 90%, the stainability of dispersed pollen was the lowest. Pollen staining viability of sugar beet lost quickly after 1 hour under the sun.