摘要
目的探讨重组人促红细胞生成素(rhEPO)对大鼠急性创伤性脑损伤的神经保护作用及其机制。方法健康雄性SD大鼠55只随机分为假手术组(n=5)、对照组(n=25)和EPO治疗组(n=25);对照组和治疗组采用改进的Feeney等的方法制作脑创伤模型,EPO治疗组在模型建立后立即腹腔注射rhEPO 5000 U/kg,对照组和假手术组在等时间点给予等量生理盐水。根据伤后处死时间点不同每组再分为5个亚组,即6 h、24 h、48 h、3 d、7 d组,每个亚组5只动物。断头、取脑,行HE染色和免疫组织化学方法检测大脑皮层组织中基质金属蛋白酶-9(MMP-9)及水通道蛋白-4(AQP-4)的表达。结果脑创伤大鼠6 h创伤灶脑组织出现MMP-9和AQP-4的表达。与假手术组比较,差异有统计学意义(P<0.05)。EPO治疗组和对照组相比较MMP-9及AQP-4的表达明显降低(P<0.05)。结论rhEPO对大鼠急性创伤性脑损伤有一定的神经保护作用,其机制可能是通过降低MMP-9及AQP-4的表达来实现的。
Objective To explore the neuroprotective mechanisms of recombinant humam erythropoietin （rhEPO） on the rats after acute traumatic brain injury. Methods Fifty-five healthy adult male SD rats were randomly dividied into sham operation group （n = 5）, control group （n = 25） and the EPO treatment group （n = 25）. The traumatic brain injury of the latter two groups was induced by Feeney＇s. rhEPO treatment group underwent intraperitoneal injection of 5000 U/kg rhEPO after the injury. The rats in the control group and the EPO treatment group were redivided into 5 subgroups （6 h,24 h ,48 h, 3 d,7 d groups） of 5 rats each respectively according to different time after the injury. The histology was detected by HE staining, the expression of MMP-9 and AQP4 was detected by immunohistochemical. Results The expression of MMP-9 and AQP-4 in the brain tissue of the injury appeared 6 hours alter trauma and was significantly higher in the injury group than that in the sham operation group （P 〈 0.05）. The expression was significantly lower in rhEPO treatment group than that in control group （P 〈 0.05）. Conclusion ReCombinant human EPO may be neuroprotective against acute traumatic brain injury in rats, probably by inhibiting the activities of MMP-9 and AQP-4.
出处
《中国现代医生》
2012年第3期4-6,共3页
China Modern Doctor
