δ阿片受体激动剂DADLE对脓毒症大鼠肝组织高迁移率族蛋白1水平的影响

Effect of delta-opioid receptor agonist DADLE on liver tissue HMGB1 level in rat with sepsis

目的研究δ阿片受体激动剂DADLE(D-Ala2-D-Leu5-enkephalin)对脓毒症大鼠肝组织高迁移率族蛋白1(HMGB1)水平的影响。方法将96只Sprague-Dawley(SD)大鼠分为假手术组(SO)、脓毒症组(SEP)、DADLE组[制模后立即给药DADLE(5mg/kg,腹腔内注)],每组32只。采用改良盲肠结扎穿孔方法(CLP)建立大鼠脓毒症模型,于制模后6 h,12 h,18 h,24 h每组各取8只处死取材,Western Blot法检测肝组织HMGB1水平。结果 12 h时SEP组和DADLE组HMGB1水平与SO组相比均明显升高(P<0.05),且DADLE组明显低于SEP组(P<0.05);18 h时SEP组和DADLE组HMGB1水平达到最高。同样DADLE组明显低于SEP组(P<0.05);24 h时SEP组和DADLE组HMGB1水平较18 h有所降低,但DADLE组仍明显低于SEP组(P<0.05)。结论 DADLE能明显降低脓毒症大鼠肝组织HMGB1水平。

Objective To study the effect of deha-opioid receptor agonist （DADLE） on liver tissue HMGB 1 level in rat with sepsis. Methods All of 96 SD rats were randomly divided in to sham operated group （SO）, septic group （SEP）, and DADLE group（DADLE）. Septic model was produced by cecal ligation and puncture （CLP）. In SO group, the abdomen was opened without any other treatment. In DADLE group, DADLE was administered at a close of 5 mL/kg by intra-peritoneal injection after CLP. Rats were sacrificed at 6 h, 12 h, 18 h and 24 h after CLP. Liver tissue HMGB1 level was determined by Westem Blot. Results Liver tissue HMGB1 levels of SEP and DADLE group began to increase at 12 h, and reached the peak around 18 h after CLP. Notably, liver tissue HMGB1 levels of DADLE group at 12 h, 18 h and 24 h were significantly low- ered than those of SEP group （P 〈 0.05）. Conclusion DADLE can significantly decrease liver tissue HMGB 1 level of septic rat.