品系和性别对小鼠甲醛实验的影响

Influence of gender and strain on formalin test using kunmlng, BALB/c and C57BL/6 Mice

目的研究品系和性别等因素对小鼠甲醛实验的影响，为选择敏感、有效的小鼠疼痛模型提供可靠的实验参考。方法在相同的实验条件下，通过给予不同品系、性别的昆明、BALB／c和C57BL／6小鼠右脚爪注射5％的甲醛溶液，记录并分析小鼠在甲醛实验早（0～5min）、晚（20～60min）时相每5分钟的舔咬爪时间。结果（1）在甲醛实验早时相，各观测时间点不同品系小鼠舔咬爪时间均无差异，而在晚时相：雄性昆明小鼠25～30min的舔咬爪时间（23．25±17．27）S明显少于C57BL／6小鼠（63．33±18．20）s；雌性昆明小鼠在20～25min舔咬爪时间（64．63±52．71）S较BALB／c小鼠（17．00±22．34）s和C57BL/6小鼠（32．17±27．42）s显著增多；C57BL／6小鼠在50～55min舔咬爪时闯（0．83±1．33）s明显比昆明小鼠（47．50±51．03）S少；（2）不同性别的小鼠甲醛实验表明，BALB／c小鼠雌雄间各观测时间点均没有显著差异。雄性昆明小鼠在25~30min的舔咬爪时间（23．25±17．27）s明显少于雌性（64．63±52．71）s；雄性C57BL／6小鼠在25～30rain的舔咬爪时闻（63．33±18．20）S明显长于雌性（22．83±18．41）s。结论品系和性别因素对小鼠甲醛实验有明显影响，为增加稳定性宜选择雄性近交系小鼠（BALB／c和C57BL／6）进行实验。

Objective To explore the effects of gender and stain on formalin test in mice. Methods Analyzing the licking/biting time of male and female Kunming,BALB/c and C57BL/6 mice in formalin test. Results （1）In the earlyphase of formalin test, there was no difference among all animal groups. In the late--phase, 20± 25 min,the licking/biting time of male Kunming mice （23.25± 17.27）s was significantly less than that of C57BL/6 （63.33± 18. 20）s, and the licking/biting time of female Kunruing mice （64.63± 52.71）s was significantly longer than that of BALB/c （17.00± 22.34）s or CATBL/6 （32. 17±27, 42）s at 20-25 min. The licking/biting time of female C57BL/6 mice at 50-55 min （0. 83± 1.33）s was significantly less than that of Kunming mice （47.50±51.03）s. （2）The licking/biting time of male Kunming mice （23. 25±17.27）s was significantly less than that of female mice （64.63±52. 71）s. The licking/biting time of male C57BL/6 mice （63.33 ±18.20）s was significantly more than that of fe- male mice （22. 83±118. 41）s. Conclusions Gender and strain could significantly affect the licking/biting time of mice in formalin test. To increase the reliability of the test,male mice should be chosen.