葎草的薄层色谱鉴别研究

Thin-Layer Chromatography of Herba Humuli Scandens

目的:建立葎草的薄层色谱鉴别方法。方法:以木犀草素对照品为对照,采用薄层色谱法进行鉴别研究。结果:供试品色谱中,在与对照品色谱相应的位置上,显相同颜色的荧光斑点,以甲苯-乙酸乙酯-甲酸(8∶2∶0.5)展开后Rf值适中,斑点清晰。结论:建立的薄层鉴别方法对葎草中木犀草素的鉴别有较好的适应性,重现性好,为完善其质量标准奠定了基础。

Objective：To establish a Thin-Layer Chromatography（TLC） identification method for Humuli Scandens.Method：Using TLC with luteolin as the reference substance.Result：Both luteolin and Humuli Scandens had the same main spot and color on the plate.The Rf value was moderate and the spot was clear by using toluence-ethyl acetate-formic acid（8∶ 2∶ 0.5） as the developing solvent substances.Conclusion：The method is adaptable and reproducible to identify luteolin of Herba Humuli Scandens,which provide a basis for the quality control of Herba Humuli Scandens.