细胞分化决定蛋白Numb／Notchl拮抗性表达与结直肠癌病理特征及预后的关系

The relationship between Numb/Notchl expression and clinical features of colorectal cancer

目的观察细胞分化决定蛋白Numb与N^chl在结直肠癌组织中的表达模式，探讨两者与结直肠癌临床病理指标及患者预后的关系。方法采用固定化蛋白质印迹法（Westernblot）及免疫组织化学回顾性检测Numb与Notchl在60例切除结直肠癌组织中的表达，分析两者表达模式与结直肠癌临床病理学指标的关系；同时研究其表达与患者生存的关系。结果结直肠癌组织中Notchl蛋白表达水平明显高于正常肠黏膜，平均为正常肠黏膜中的2．60倍（43／60比17／60，P〈0．01）。而Numb蛋白表达水平明显低于正常肠黏膜，平均为正常肠黏膜的0．52倍（39／60比21／60，P〈0．01）。N^chl表达水平与Numb表达呈显著负相关（r=-0．782，P〈0．05）。同时两者表达与结直肠癌的分化程度、淋巴结转移、TNM分期密切相关。Notchl高表达的患者（43例）预后明显差于Notchl低表达表达的患者（17例，P〈0．05），而与之相反的是Numb低表达（39例）预后差于Numb高表达患者（21例），差异有统计学意义（P〈0．05）。结论Numb与Notch拮抗性表达可能与结直肠癌发生发展密切相关，可作为结直肠癌某些生物学行为及判定预后的新的参考指标。

Objective The purpose of this study was conducted to investigate the relationship be- tween the expression of Numb/Notchl and the clinicopathological parameters and prognosis in colorectal cancer. Methods Numb and Notchl protein expression levels in 60 cases of resected colorectal cancer were detected by Western blotting method, the relations between Numb and Notchl expression levels and the survisal periods were estimated by Kaplan-Meier curve. Results Notchl expression level were in- creased in colorectal cancer compared with adjacent normal mucosa, the median values were 2. 60-fold （43/60 vs 17/60, P 〈0. 01 ）. However Numb expression level were decreased in colorectal cancer com- pared with adjacent normal mucosa, the median values were 0. 52-fold （39/60 vs 21/60, P 〈 O. 01 ）. The correlation between Notchl and Numb protein level are significant at the 0. 01 level （r = -0. 782, P 〈 0. 05 ）. It was found that expression of Notchl and Numb was related to differentiation, lymph nodes metas- tasis and TNM staging in colorectal cancer （P 〈 0. 05 ）. Patients with Notchl high-expression （43/60） and Numb low-expression （39/60） had worse prognosis than those with Notchl low-expression （17/60） and Numb high-expression （21/60） in colorectal cancer patients （P 〈 0. 05 and P 〈 O. 05 ）. Conclusion Our results show that Numb and Notchl antagonistic expression are close related to colorectal cancer develop- ment, which can be a couple of novel markers for diagnosing certain biological behaviours and predicting prognosis in colorectal ancer.