肾脏远隔缺血后适应在兔急性肠系膜缺血再灌注损伤中的作用

The study of renal ischemia remote postconditioning intervention in the rabbits acute mesenteric ischemia-reperfusion injury model

目的观察以肾脏作为远隔器官的远隔缺血后适应能否减轻兔急性肠系膜缺血再灌注损伤。方法将雄性新西兰兔90只随机分为对照组、缺血再灌注（I／R）组、远隔缺血后适应（IRpostC）组，每组30只。分别干预后采集各组兔部分肠道组织作为标本，试剂盒检测肠道组织内丙二醛（MDA）、髓过氧化物酶（MPO）水平，苏木素一伊红（HE）染色，chiu6级评分法观察肠黏膜损伤，电镜下观察肠上皮细胞变化。结果I／R组肠道组织中MDA、MPO测定值及肠黏膜损伤评分分别为（14．72±O．21）nmol／mg、（1．65±0．35）U／g、（3．30±0．69），IRpostC组分别为（7．134-0．40）nmol／mg、（0．91±0．33）U／g、2．10±0．92，与对照组[（3．28±0．26）nmol／mg、（O．52±0．23）U／g、0．69±0．52]比较差异均有统计学意义（P〈0．01）。与I／R组比较，IRpostC组肠道组织中MDA、MPO水平及肠黏膜损伤评分明显降低（P〈0．01），电镜下发现细胞损伤减轻。结论以肾脏作为远隔器官的远隔缺血后适应可明显降低兔急性肠系膜缺血再灌注损伤后肠道组织中MDA、MPO水平及肠黏膜损伤。

Objective To study the effect of ischemia remote postconditioning （renal） intervention in the rabbits acute mesenteric ischemia-reperfusion injury model. Methods 90 rabbits were divided randomly into Con, I/R, IRpostC group （n = 30）. I/R models were established by clamping-releasing the superior mesenteric artery. To detect the levels of malondialdehyde （MDA） and myeloperoxidase （MPO） in intestinal tissues by kit. To determin the pathology score of damaged intestinal mucosae by chiu-6 standared. The damage of intestinal epithelial cells were observed by electron microscopy. Results The MDA, MPO levels and pathology score in intestinal tissues was （ 14. 72±0. 21 ） mnol/mg, （ 1.65 ±0. 35 ） U/g, 3.30±0. 69 respectively in I/R group, and in IRpost C group was （7. 13±0. 40） nmol/mg, （0. 91±0. 33） U/g, 2. 10±0. 92 respectively. There were increased obviously compared with the Con group [ （3.28±0.26） nmol/mg, （0. 52±0. 23） U/g, 0. 69±0. 52]. Compared with the I/R group, the MDA, MPO levels and pathology score in intestinal tissues were decreased obviously in the IRpostC group （P 〈 0. 01 ）. Cells damage were reduced significantly in IRpostC group compared with the I/R group by electron microscope. Conclusion The acute mesenteric ischemia-reperfusion injury were reduced by ischemia remote postconditioning （renal） intervention in rabbits.