p16、ESRβ蛋白在HPV相关宫颈癌中的表达及意义

Expressions and significances of p16 protein and estrogen receptor β protein in human papillomavirus-related cervical cancer

目的:探讨p16、ESRβ蛋白在HPV相关宫颈癌中的表达及临床意义。方法:采用表面等离子谐振法检测90例宫颈癌中感染HPV类型及载量,用免疫组化方法分别检测90例宫颈癌和30例对照组宫颈组织中p16、ESRβ蛋白的表达并分析结果。结果:HPV感染类型以51亚型为主,但16亚型和18亚型的平均载量较高,与其他类型相比差异有统计学意义(P<0.05);p16蛋白的表达在宫颈癌组和对照组中的阳性率分别为100.00%和20.00%,差异有统计学意义(P<0.05);ESRβ蛋白表达的阳性率分别为14.44%和76.67%,差异有统计学意义(P<0.05)。结论:HPV、p16、ESRβ在宫颈癌的发生发展中均有重要作用,在临床工作中检测三者指标均有助于宫颈癌的诊断和预后。

Objective： To explore the expressions and significances of p16 protein and estrogen receptor β protein in human papillomavirus （HPV） - related cervical cancer. Methods： Surface plasma resonance method was used to detect the types and loads of HPV infection in 90 cases with cervical cancer, immunohistochemical method was used to detect the expression levels of p16 protein and estrogen receptor β protein in 90 cases with cervical cancer （cervical cancer group） and 30 cases with normal cervical tissue （control group）, then the results were analyzed. Results： The main type of HPV infection was HPV 5 l subtype, but the average loads of HPV 16 subtype and HPV 18 subtype were higher, compared with other HPV subtypes, there was significant difference （P 〈 0. 05 ） . The positive expression rates of p16 protein in cervical cancer group and control group were 100. 00% and 20. 00% , respectively, there was significant difference between the two groups （P 〈 0. 05 ） ; the positive expression rates of estrogen receptor β protein in cervical cancer group and control group were 14. 44% and 76. 67% , respectively, there was significant difference between the two groups （P 〈 0. 05） . Conclusion： HPV, p16 protein, and es- trogen receptor 13 protein play important roles in th occurrence and development of cervical cancer, the detections of the three indicators are helpful to diagnosis and prognosis of cervical cancer in clinic.