磁性纳米铁联合多柔比星对Raji细胞的影响

Effect of combination with magnetic nanoparticle of Fe3O4 and adriamycin on Raji cell line

目的探讨磁性纳米Fe3O4颗粒（Fe，OrMNP）联合多柔比星（ADM）对Raji细胞的影响。方法采用MTT法检测细胞的增殖，锥虫蓝染色计数细胞的活性，流式细胞术检测细胞凋亡情况，Westemblot检测p53和NF．KB的表达水平。结果ADM及Fe，04-MNP—ADM对Raji细胞的生长抑制率与药物浓度、作用时间呈正相关（r=0．412，P=0．027；r=0．523，P=0．014）；12、24、48h细胞凋亡率二者分别为8．76％比14．85％、35．08％比44．50％、44．00％比69．40％，差异有统计学意义（t值分别为-9．137、-4．808、-6．337，P值分别为0．012、0．041、0．024）；Western blot检测示ADM组与Fe304-MNP—ADM组NF．KB蛋白的灰度条带与内参比较分别为4．22-4-0．32、3．31-4-0．28，p53蛋白的条带灰度值分别为1．042±0．114、1．270±0．091，差异具有统计学意义（t=-54．416，P=0．035；t=33．963，P=0．047）。结论Fe30，MNP联合ADM能够有效抑制Raji细胞增殖并诱导其凋亡，作用机制可能与增强p53蛋白活化、抑制NF—KB通路的激活有关。

Objective To investigate the effect of combination with magnetic nanopartiele of Fe3O4 and adriamycin （ADM） on Raji cell line. Methods Raji cells were cultured with Fe3O4-magnetic nanoparticle and ADM after using mechanical absortion polymerization, and the cell viability was detected by MTT and trypan-blue exclusion. The apoptosis was detected by flow cytometry, and the expression of p53 and NF-KB were measured by Western blot. Results Raji cell proliferation ratio was significantly inhibited in concentration-and time-dependent manners in both ADM and Fe3Oa-MNP-ADM groups （r =0.412, P =0.027; r =0.523, P =0.014）. The percentages of apoptotic ceils induced by ADM and Fe304-MNP-ADM were 8.76 % vs 14.85 %, 35.08 % vs 44.50 %, 44.00 % vs 69.40 % in 12, 24 and 48 hours, respectively （t = -9.137, -4.808, -6.337; P =0.012, 0.041, 0.024）. The grey straps of NF-KB measured by Western blot were 4.22± 0.32, 3.31±0.28 in ADM and Fe304-MNP-ADM group （t = -54.416, P =0.035）, whereas the grey straps of p53 were 1.042±0.114, 1.270±0.091, respectively （t = 33.963, P =0.047）. Conclusion Combination therapy of Fe304-MNP and ADM could inhibit proliferation and induce apoptosis of Raji cells line, which may due to up- regulation of p53 and down-regulation of NF-KB.