利用SRAP标记分析黄淮海地区纤毛鹅观草居群的遗传多样性

GENETIC DIVERSITY OF ROEGNERIA CILIARIS POPULATIONS IN THE HUANG- HUAI- HAI REGION ANANLYZED USING SRAP MOLECULAR MARKERS

笔者利用SRAP标记对纤毛鹅观草29个居群进行了遗传多样性分析．本实验利用11对能够在不同居群中扩增出稳定多态性条带的引物进行扩增，结果表明，平均每对引物获得9．34个多态性位点，多态位点百分率为80．47％．G5值在0．5833到0．9677之间，平均GS值为0，7755，这表明，居群之间差异明显，具有较为丰富的遗传多样性．对所有居群进行聚类，可聚为6类，其中大部分来自相同或相似生态地理环境的居群聚为一类；对不同生态地理类群基于Nei氏无偏估计的遗传一致度的聚类分析表明，各生态地理类群间的遗传分化与其所处的生态地理环境具有一定的相关性；而UPGMA聚类分析结果表明SRAP标记技术能较真实地反映纤毛鹅观草居群间的亲缘关系，可以用于纤毛鹅观草遗传多样性研究．

Employing Sequence -related amplified polymorphism （SRAP） molecular markers, the genetic diversity of 29 wild accessions of Roegneria ciliaris （Trin.） Nevski was analyzed. The results indicated that, after PCR with 11 pairs of primer which could well show polymorphic bands, every pair of primer gained an average 9.34 polymorphic bands and the percentage of polymorphic bands in average was 80.47 %. In addition, the Nei ＇ s genetic similarity coefficient of the tested accessions ranged from 0. 583 3 to 0. 967 7 with the average Nei＇ s coefficient 0. 775 5. These results suggested that there was rich genetic diversity among the wild resources of R. ciliaris tested. Moreover, these wild accessions were clustered into six populations and those from the same origin were frequently clustered into one group. Plus, data analyzed based on Nei＇ s unbiased measures of genetic identity, indicated that there was a definitely link between genetic differentiation and eco -geographical habitats among different populations meanwhile results of UPGMA cluster analysis showed that SRAP molecular markers could so effectively and efficiently reflect the relationships among the different populations of R. ciliaris that it could be used to study the genetic diversity of R. ciliaris.