摘要
维生素 D3 (VD3 ) proliposomes (VDP ) ，由 hydrogenated phosphatidycholine (HPC ) 和 VD3 组成了，用 supercritical 反溶剂技术(SAS ) 被准备。在 VDP 装载的 VD3 上的操作条件(温度，压力和部件) 的效果被学习。以 8.0 MPa 的压力的最佳条件， 45 湥 ?慢敳 d 的温度 ??? 晸？？
Vitamin D3 （VD3） proliposomes （VDP）, consisted of hydrogenated phosphatidycholine （HPC） and VD3, were prepared using supercritical anti-solvent technology （SAS）. The effects of operation conditions （temperature, pressure and components） on the VD3 loading in VDP were studied. At the optimum conditions of pressure of 8.0 MPa, temperature of 45 ℃, and the mass ratio of 15.0% between VD3 and HPC, the VD3 loading reached 12.89%. VD3 liposomes （VDL） were obtained by hydrating VDP and the entrapment efficiency of VD3 in VDL reached 98.5%. The morphology and structure of VDP and VDL were characterized by SEM （scanning electron micro-scope）, TEM （transmission electron microscope） and XRD （X-ray diffractometer）. The structure of VD3 nanoparti-cles in HPC matrix was formed. The size of VDL with an average diameter of about 1μm was determined by dynamic light scattering instrument （DLS）. The results indicated that VDP can be made by SAS and VDL with high entrapment efficiency can be formed easily via the hydration of VDP.
基金
Supported by the National High Technology Research and Development Program of China （2007AA 10Z350） and the National Natural Science Foundation of China （20976103）.
