nHACP/CF支架材料对人脐静脉内皮细胞早期附着生长影响的研究

The impact of nHACP/CF scaffold material on the early adhesion growths of human umbilical vein endothelial cells

目的探讨甲壳素纤维增强多孔胶原基骨组织工程支架材料(nHACP/CF)对人脐静脉内皮细胞(humanumbilical vein endothelial cells,hUVEC)早期附着生长情况的影响。方法本研究于2008年8月至2010年1月在中国医科大学实验病理教研室实验室进行。体外培养hUVEC,并与nHACP/CF支架材料联合培养,观察其在支架上的生长情况,通过倒置显微镜、扫描电子显微镜以及HE染色等方法观察细胞在nHACP/CF支架材料上的早期附着情况。结果 hUVEC在nHACP/CF支架材料上生长良好。结论 nHACP/CF支架材料有利于hU-VEC的早期黏附、生长,具有良好的细胞相容性,是一种很有前景的骨组织工程支架材料。

Objective To study the impact of nHACP / CF scaffold material on the early adhesion growths of human um-bilical vein endothelial cells （hUVEC）. Methods hUVEC were seeded on nHACP / CF scaffold material and cultured in vitro. The culture process was observed by inverted phase contrast microscope, SEM and HE staining to study the effect of nHACP / CF scaffold material on hUVEC. Results The spreading and adherence of hUVEC throughout the inner--pores of the scaffold material were observed. Conclusion The nHACP / CF scaffold material contributes to the spreading and adherence of hUVEC and it can be used as bone tissue engineering scaffold material.