多叶棘豆组织培养及快速繁殖研究

Study on Tissue Culture and Rapid Propagation of Oxytropis myriophylla

目的:由于多叶棘豆的种子为短寿型,本文通过组织培养来确定多叶棘豆快速繁殖的人工繁殖方法。方法:将种子接种于诱导培养基上,待其长成小苗后用不定芽作为外植体,在MS培养基上添加不同种类及不同浓度的激素,对多叶棘豆进行丛生芽诱导及根的培养。结果:丛生芽诱导的适宜培养基为MS+0.5 mg/L 6-BA+0.2mg/L NAA;生根壮苗的适宜培养基为1/2MS+NAA 0.1 mg/L。结论:采用组织培养方式可进行多叶棘豆的快速繁殖。

Objective：To study the plant tissue culture and rapid proagation of O.myriophylla.Methods： The adventitious buds which were from the seedlings germinated from the seeds on the initial medium were taken as explants.These explants were cultured on MS culture media by adding different portions of hormones at various conditions.Results： The proper initial medium for the adventitious buds was MS＋6-BA 0.5 mg/L＋NAA 0.2 mg/L,and the best medium for rooting was 1/2MS＋NAA 0.1 mg/L.Conclusion： Tissue culture of O.myriophylla could make its propagation rapidly.