甘肃产不同品种藏药绿绒蒿的质量评价方法研究

Study on Quality Control Methods for Different Varieties of Meconopsis of Gansu

目的:建立藏药绿绒蒿中槲皮素HPLC含量测定及TLC鉴别方法,评价甘肃产不同品种绿绒蒿质量;同时以快速鉴别为目的初步研究绿绒蒿药材可见-紫外光谱特征。方法:采用HPLC法测定水解物中总槲皮素含量,TLC法进行鉴别;采用分光光度法测定绿绒蒿药材不同溶剂提取物可见-紫外光谱。结果:酸水解条件:盐酸-80%甲醇(8∶25)90℃水浴水解1.5 h。色谱条件:色谱柱:Hypersil-Keystone-C18(250 mm×4.6 mm,5μm);流动相:甲醇-水(50∶50);检测波长:360 nm;流速:1.0 mL/min;柱温:35℃;槲皮素进样量在0.01μg～0.12μg范围内与峰面积呈良好线性关系(r=0.9997);平均回收率98.83%,RSD=1.69%(n=9)。甘肃产全缘绿绒蒿、五脉绿绒蒿中总槲皮素含量分别为0.1135 mg/g和0.0842 mg/g。结论:本实验确定的酸水解条件、槲皮素HPLC含量测定方法及TLC鉴别方法简便易行,结果可靠,为评价甘肃绿绒蒿药材质量提供参考;同时对其不同溶剂提取物可见-紫外光谱特征的研究将为药材的快速鉴别提供参考。

Objective：To establish the quantitative determination method and TLC identification of Meconopsis and study the Vis-UV spectrum character for fast identification.Methods： Meconopsis were identified by TLC,and the content of quercetin was determined by HPLC.Results： The acid hydrolyze condition was HCl-80%MeOH（8∶25）90 ℃ calefaction by water for 1.5 hour.The condition of HPLC ： Hypersil-Keystone-C18（250 mm×4.6 mm,5 μm）column,MeOH-Water（50∶50）as mobile phase,detection wavelength at 360nm,flow rate at 1.0 mL/min,column temperature at 35 ℃,quercetin showed a good linear relationship at a range of 0.01～0.12μg,r=0.9997.The average recovery was 98.83%,and RSD was 1.69%（n=9）.The contents of quercetin in Meconopsis integrifolia and Meconopsis quintuplinervia of Gansu were 0.1135 mg/g and 0.0842 mg/g respectively.Conclusion： The method is simple,accurate and specific.It can be used for the quality control and fast identification of Meconopsis.