血浆(1-3)-β-D-葡聚糖检测对临床侵袭性真菌感染诊断价值的研究

Research on(1-3)-β-D-glucan detection in plasma to diagnose invasive fungal infection

目的对临床疑似侵袭性真菌感染(IFI)患者进行血浆(1-3)-β-D-葡聚糖检测和真菌培养鉴定结果比较,评估血浆(1-3)-β-D-葡聚糖检测诊断IFI感染的实用价值。方法对疑似IFI感染患者131例抽取静脉血分离血浆,检测(1-3)-β-D-葡聚糖;于发热时采静脉血做真菌培养鉴定,并进行分析。结果 131例疑似IFI患者血浆(1-3)-β-D-葡聚糖阳性率为41.22%,真菌培养阳性率为37.40%。2种方法检测阳性率差异无统计学意义(P>0.05)。以真菌培养结果为参照,血浆(1-3)-β-D-葡聚糖检测敏感性为95.92,特异性为91.46,阳性预测值为87.04,阴性预测值为97.40。结论血浆(1-3)-β-D-葡聚糖检测具有较高的敏感性和特异性,对临床IFI的诊断具有实用价值。

Objective To evaluate the practical value of invasive fungal infection（IFI） with the（1-3）-β-D-glucan detection in plasma by comparing the results of（1-3）-β-D-glucan detecting and those of a conventional fungus culture for the patients who were suspected of having IFI.Methods 131 samples were collected from patients suspected of having IFI,venous blood was taken and plasma was separated.The level of（1-3）-β-D-glucan in plasma was determined and a conventional fungus culture was done using venous blood taken at durante febre.Results The positive rate of 131 samples suspected of having IFI was 41.22%,the positive rate of fungal culture was 37.40%,and the difference of two methods detected for IFI without significant statistically（P0.05）.Taked the conventional fungus culture as a reference,the plasma（1-3）-β-D-glucan detection had a sensitivity of 95.92,specificity of 91.46,positive predictive value of 87.04,and negative predictive value of 97.40.Conclusion The level of（1-3）-β-D-glucan in plasma has highly of sensitive and specific,and this new method of testing can be used to diagnose IFI in clinical.