摘要
超数排卵在动物胚胎工程技术的体内胚生产中起关键作用,不同品种甚至个体对超数排卵的反应不同。人绝经期促性腺激素(HMG)是从绝经期妇女尿液中提纯得到的具有促卵泡素(FSH)活性的蛋白质激素,在医学上广泛应用于诱导排卵和治疗不育不孕症。本试验以安格斯青年母牛为超排供体,采用分次肌肉注射激素方法进行超排处理,比较了HMG与FSH用于安格斯母牛超数排卵的效果。B超检查发现,HMG和FSH超排处理后牛只两侧卵巢上直径>1cm的卵泡数分别为10.33±0.52和9.25±0.46个,二者差异不显著(P>0.05),表明HMG与FSH同样安全有效;总剂量1 400IU的HMG或FSH用于安格斯母牛超排后B超检查卵巢没有发现异常变化,说明该剂量是用于安格斯母牛超数排卵的安全有效剂量。
Superovulation plays an important role in iv vivo production of embryos in the embryo engineering techniques, and is affected by animal breeds and even individuals. Human menopausal gonadotrophin（HMG）is a protein hormone refined from urine of menopausal women, which has high follicle stimulating hormone （FSH） activity. The aim of this study is to compare the effects of HMG and FSH in superovulation regimens via multiple intramuscular injections in Angus heifers. B type ultrasonography was performed to evaluate superovulation treatment. The results showed that there was no significant difference between HMG and FSH （10.33±0.52 vs 9.25±0.46, P〉0.05, respectively） in the number of follicles on both ovaries. All animals responded well to the superovulatory stimulus by a total dose of 1400IU HMG or FSH, indicating that this dose is safe and effective for superovulation in Angus Heifers.
出处
《中国奶牛》
2012年第1期17-20,共4页
China Dairy Cattle
基金
现代农业技术体系(项目编号:CARS-37-04B)
公益性行业(农业)科研专项课题(项目编号:201003060)资助
