西洋参超微粉和普通粉的溶出度及药效学对比研究

The Comparison Study on Dissolution and Pharmacodynamics Effects between Submicron and Normal Powder of American Ginseng

目的:对比不同粒径的西洋参粉末的溶出度、药效学的区别。方法:采用电子显微镜扫描,记录西洋参超微粉的粉体特征和细胞破碎照片,测定西洋参超微粉和普通粉的平均粒径;超声提取对比西洋参超微粉和普通粉收得率,高效液相色谱法测定超声提取西洋参超微粉皂苷类成分指纹图谱和人参皂苷Rb1、Rg1、Re的溶出度;动物试验对比口服西洋参超微粉和普通粉的小鼠耐缺氧实验耐缺氧存活时间,动物试验对比口服西洋参超微粉和普通粉末的小鼠低温游泳试验低温游泳时间。结论:超微粉碎技术可以提高西洋参的细胞破壁程度,提高了药物的生物利用度,增加有效成分溶出度,提高药材的功效。

Objective：To compare the dissolution and pharmacodynamics effects on different grain sizes of American ginseng powder.Methods： The powder characterization were measured by scanning electron microscope;the saponins fingerprint and the dissolutions of ginsenoside Rb1,Rg1 and Re were investigated by HPLC;the hypoxia and hypothermy tolerance tests on mice were performed to compare the pharmacodynamics effects between submicron and normal powder of American ginseng.Results： In the submicron American ginseng powder,the crashed cells made the bioactive compounds easier to dissolute,and improved the mice performance in hypoxia and hypothermy tolerance tests.Conclusion： The submicron powder has better bioavailability and pharmacodynamics effect than the normal size of American ginseng powder.