锌酞氰介导的光动力学疗法对小鼠Lewis肺癌细胞的影响研究

Study of effect of ZnPc-PDT on mice Lewis lung cancer cells

目的:初步探讨锌酞氰(ZnPc)介导的光动力学疗法(ZnPc-PDT)对小鼠Lewis肺癌细胞的杀伤作用,探索ZnPc-PDT杀伤Lewis肺癌细胞的最佳工作参数。方法:通过分光光度计测量ZnPc的吸收峰值,选取670 nm的半导体激光器作为激发光源;分别利用MTT法和流式细胞术检测光敏剂浓度、光照时间及激光功率密度对ZnPc-PDT对Lewis肺癌细胞的杀伤能力的影响。结果:ZnPc的吸收峰值为670 nm;ZnPc-PDT对肺癌细胞的杀伤作用均随光敏剂浓度、光照时间、光源功率密度的增强而增强,但增大到一定值后会趋于饱和。结论:ZnPc组分单一,与其相对应的吸收波长对组织的穿透能力较强;ZnPc介导的PDT能有效杀伤小鼠Lewis肺癌细胞,杀伤效果主要受光敏剂浓度、光照时间及光源功率密度等参数的影响。

Objective： To investigate the effect of ZnPc-PDT on mice Lewis lung cancer cells and explore the best parameters of ZnPc-PDT for lung cancer.Methods： We used the ultraviolet spectrophotometer to measure the absorption peak values and selected 670nm semiconductor lasers as the excitation light resources.Meanwhile,we employed MTT and FACS to observe the killing effects of ZnPC-PDT on Lewis lung cancer cells under different photosensitizers’ concentration,exposure time,and different laser power density.Results： The absorption peak values of ZnPc was 670nm,and the effects of killing lung cancer increased with the increasing of photosensitizers’ concentration,exposure time,and different light source power density,while the effects would be saturated after reaching certain value.Conclusion： ZnPc is a single components material,and its absorption wavelength has strong penetration ability to organization.ZnPc-PDT could effectively induce the death of Lewis lung cancer cells in mice.The key factors of effects of killing lung cancer are photosensitizers’ concentration,exposure time,and different laser power density.