成像检测在DNA毛细管电泳中的应用

Imaging Detection Applied in DNA Capillary Electrophoresis

本文研究了成像检测方式以及阵列检测器在DNA毛细管电泳的荧光检测中的应用,目的在于减小检测过程引起的样品区带增宽,提高DNA毛细管电泳的分辨率和分离速度。依据分离度理论以及模拟计算结果,归纳了检测宽度的影响规律,指出减小检测宽度对于高效分离,特别是高速微流控系统电泳分离的重要性。实验测量并比较了成像检测与传统光强检测的结果。提出阵列检测器的信号增强方法,并进行了模拟验证。研究表明,成像检测可显著减小毛细管电泳的检测宽度,提高分离效率和分离速度。

Imaging detection and Charge Coupled Device（CCD） detector was adopted in fluorescence detection of DNA capillary electrophoresis in order to decrease the detection width and improve the efficiency and speed of DNA separation.The importance of small detection width for high-speed capillary electrophoresis,especially the separation performed in micro-fluid device,was illustrated according to theoretical calculation based on resolution theory of capillary electrophoresis.The performances of imaging detection and conventional intensity detection were measured and compared.The method of data processing to intensify the DNA band signal of CCD detector was proposed,and verified by simulation.Imaging detection and its data processing method can remarkably decrease the detection width of capillary electrophoresis,and then improve the efficiency and speed of DNA separation.