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RegⅠ-shRNA载体对胃泌素促胃癌细胞BGC823增殖作用的影响 被引量：2

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摘要 目的探讨再生蛋白Ⅰ(RegⅠ)在胃泌素刺激胃癌细胞增殖通路中的作用。方法构建3个RegⅠ-shRNA表达载体:pSUPER-EGFP-REG/1、pSUPER-EGFP-REG/2和pSUPER-EGFP-REG/3。分别转染胃癌细胞BGC823,G418筛选。RT-PCR检测RegⅠmRNA的转录水平,建立RegⅠ基因表达抑制胃癌细胞系。MTT法检测胃泌素孵育对胃癌细胞增殖的效应。结果 pSUPER-EGFP-REG/1可有效抑制RegⅠmRNA在胃癌细胞BGC823中的表达。胃泌素孵育前,RegⅠ基因表达抑制的胃癌细胞增殖能力较无基因表达抑制的胃癌细胞明显降低(P<0.05);胃泌素孵育后,胃癌细胞的增殖能力明显增加(P<0.05),而RegⅠ基因表达抑制的胃癌细胞增殖能力无明显改变(P>0.05)。结论成功建立了RegⅠ基因表达抑制的胃癌细胞系,RegⅠ基因可能对胃泌素促胃癌细胞的增殖有促进的作用。
作者 程珊 丁一 张钦宪
机构地区 新乡医学院基础医学院组织胚胎学教研室 郑州大学基础医学院组织胚胎学教研室
出处 《广东医学》 CAS CSCD 北大核心 2012年第1期65-67,共3页 Guangdong Medical Journal
关键词 RegⅠ基因 胃泌素 RNA干扰
分类号 R73-36 [医药卫生—肿瘤]
  • 相关文献

参考文献11

  • 1SMITH K A, PATEL O, LACHAL S, et al. Production, secretion, and biological activity of the C - terminal flanking peptide of human progastrin[J]. Gastroenterology, 2006, 131(5): 1463-1474.
  • 2DHAR D K, UDAGAWA J, ISHIHARA S, et al. Expression of regenerating gene I in gastric adenocarcinomas: correlation with tumor differentiation status and patient survival [ J]. Cancer, 2004, 100(6) : 1130-1136.
  • 3FUKUI H, FUJII S, TAKEDA J, et al. Expression of reg I alpha protein in human gastric cancers [ J ]. Digestion, 2004, 69 ( 3 ) : 177 - 184.
  • 4HUR K, KWAK M K, LEE H J, et al. Expression of gastrin and its receptor in human gastric cancer tissue[J]. J Cancer Res Clin Oncol, 2006, 132(2) : 85 -91.
  • 5LEE H K, LEE H J, HUR K, et al. Growth effect of gastrin on gastric cancer and its clinical implications for gastric cancer surgery [J]. Oncol Rep, 2005, 14(2) : 383 -388.
  • 6王霞,魏大鹏,蔡美英.胃泌素在胃癌细胞株的表达及抗胃泌素McAb对胃癌细胞株生长的影响[J].中华肿瘤杂志,2005,27(5):276-278. 被引量：10
  • 7SLICE L W, HEDIKIAN R, ZHUKOVA E, et al. Gastrin and EGF synergstically induce cyclooxygenase - 2 expression in Swiss3T3 fibroblasts that express the CCK 2 receptor [ J ]. Cell Physiol, 2003, 196(3) : 454 -463.
  • 8TAKAISHI S, WANG T C. Gene expression profiling in a mouse model of Helicobacter - induced gastric cancer [ J ]. Cancer Sci, 2007. 98 ( 3 ) : 284 - 293.
  • 9SEKIKAWA A, FUKUI H, FUJII S, et al. REG I alpha protein mediates an anti - apoptotic effect of STAT3 signaling in gastric cancer cells [ J ]. Carcinogenesis, 2008, 29 ( 1 ) : 76 - 83.
  • 10FUKUI H, KINOSHITA Y, MAEKAWA T, et al. Regenerating gene protein may mediate gastric mucosal proliferation induced by hypergastrinemia in rats [ J ]. Gastroenterology, 1998, 115 ( 6 ) : 1483 - 1493.

二级参考文献25

  • 1McWilliams DF,Watson SA,Crosbee DM,et al.Coexpression of gastrin and gastrin receptors (CCK-B and delta CCK-B ) in gastrointestinal tumour cell lines. Gut, 1998, 42: 795-798.
  • 2Smith JP, Shih A, Wu Y, et al. Gastrin regulates growth of human pancreatic cancer in a tonic and autocrine fashion. Am J Physiol, 1996,270: R1078-1084.
  • 3Caplin ME, Khan K, Grimes S, et al. Effect of gastrin and anfi-gastrin antibodies on proliferation of hepatocyte cell lines. Dig Dis Sci, 2001,46: 1356-1366.
  • 4Takayama T, Sekine T, Makuuchi M, et al. Adoptive immunotherapy to lower postsurgical recurrence rates of hepatocellular carcinoma: a randomised trial, lancet, 2000, 356: 802-807.
  • 5Rozengurt E, Walsh JH. Gastrin, CCK, signaling, and cancer. Annu Rev Physiol, 2001, 63:49-76.
  • 6Watson SA, Clarke PA, Morris TM, et al. Antiserum raised against an epitope of the cholecystokinin B/gastrin receptor inhibits hepatic invasion of a human colon tumor. Cancer Res, 2000,60:5902-5907.
  • 7Brett BT, Smith SC, Bouvier CV, et al. Phase Ⅱ study of anti-gastrin-17 antibodies, raised to G17DT, in advanced pancreatic cancer. J Chin Oncol, 2002,20:4225-4231.
  • 8Yoshikazu Kinoshita,Shunji Ishihara,Yasunori Kadowaki,Hiroshi Fukui,Tsutomu Chiba.Reg protein is a unique growth factor of gastric mucosal cells[J]. Journal of Gastroenterology . 2004 (6)
  • 9RIVERA F,VEGA-VILLEGAS M E,LOPEZ-BREAM F.Chemotherapy of advanced gastric cancer.. Cancer Treatment Reviews . 2007
  • 10KINOSHITA Y,ISHIHARA S,MIYAOKA Y,etal.Reg protein is overexpressed in gastric cancercells,where it activates a signal transductionpathway that converges on ERK1/2 to stimulategrowth. FEBS Letters . 2002

共引文献9

同被引文献21

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二级引证文献17

广东医学
2012年 第1期

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