日立F-4600型荧光分光光度计测定硒含量方法的研究

Research of Trace Element Selenium Contents by Hitachi F-4600 Fluorescence Spectrophotometer

目的:采用日立F-4600型荧光分光光度计研究测定硒含量的方法。方法:采用荧光法测定硒含量。结果:按实验所建立方法测定貉血清、被毛、心、肝、脾、肺、肾、肌肉中硒含量,发现均在0.5μg范围内变化,且与荧光强度呈线性关系。结论:应用日立F-4600型荧光分光光度计建立荧光法测定硒含量的方案是可行的。

Objective： To research the way of determining selenium contents by Hitachi F- 4600 fluorescence spectrophotometer. Methods： Selenium contents were determined by fluorospectrophotometry. Results： Selenium contents of serum, fur, heart, liver, spleen, lung, kidney and muscle were determined by this way in raccoon dog. Conclusion： The scheme of determining selenium contents by Hitachi F - 4600 fluorescence spectrophotometer was feasible.