罗格列酮对重症急性胰腺炎合并肺损伤的防治作用

The role of resisitin in the prophylactic and therapeutic treatments of rosiglitazone in rats with severe acute pancreatitis

目的研究抵抗素在过氧化物酶增殖物激活受体（PPAR-γ）激动剂（罗格列酮）对大鼠重症急性胰腺炎（SAP）及其合并肺损伤防治中的作用及其作用机制。方法用ELISA法检测大鼠血清淀粉酶（AMY）、抵抗素、肿瘤坏死因子（TNF-α）、白细胞介素-1β（IL-1β）和c-反应蛋白（CRP）的水平变化，检测肺组织髓过氧化物酶（MPO）、肺湿／干重比值、胰腺／体质量比值、胰腺和肺组织病理变化；免疫组化检测胰腺组织中抵抗素的表达；实时定量PCR法检测胰腺组织中抵抗素mRNA的水平。结果罗格列酮预防组和治疗组大鼠血清AMY、抵抗素、TNF-α、IL-1β和CRP水平较SAP组均明显下降（均P〈0．01），预防组和治疗组与对照组相比均有明显升高（均P〈0．01），预防组和治疗组之间相比较有所变化，但两者之间元统计学意义；罗格列酮预防和治疗组胰腺／体质量比、胰腺病理评分、肺组织MPO含量和肺的病理评分较SAP组均明显降低（均P〈0．01）；对照组、SAP组、预防组和治疗组大鼠的胰腺组织抵抗素mRNA相对表达量（RQ值）较SAP组明显降低（均P〈0．01），而与对照组相比较均明显升高（P〈0．01），但预防组与治疗组之间差异无统计学意义。结论罗格列酮对SAP及合并肺损伤有明显的预防和治疗作用。

Objective To study the role and mechanism of resisitiu in prophylactic and therapeutic treat- ments of rosiglitazone, a specific peroxisome proliferator-activated receptor-（PPARγ） ligand, in rats with severe acute pancreatitis （SAP）and pancreatitis-associated pulmonary injury. Methods The levels of amylase （AMY）, Resistin, TNF-α, IL-1β and C reactive protein （CRP） in blood plasma, lung myeloperoxidase （ MPO ） activity, pancreas/body weight ratio and lung wet/dry weight ratio were evaluated. Pancreatic and pulmonary pathology were observed. The ex- pression of resistin in pancreas was detected by immunohistochemistry. The gene expression of resistin mRNA was in- vestigated by real-time PCR. Results Both prophylactic and therapeutic treatments with rosightazone could obviously ameliorate the levels of AMY, resistin,TNF-α, IL-1β and CRP （ all P 〈 0.01 ）. Compared with the control group,both prophylactic and therapeutic treatment groups were higher（ all P 〈 0.01 ）. The prophylactic treatment group was not different from the therapeutic treatment group. Both prophylactic and therapeutic treatments with rosiglitazone could significantly reduce pancreas/body weight ratio, pancreatic pathology, MPO, pulmonary pathology （ all P 〈 0. 01 ）. Compared with the SAP group, the expression of resistin mRNA in the prophylactic and therapeutic treatment groups were obviously decreased. Conclusion Rosiglitazone could obviously ameliorate pancreatitis and pulmonary injury induced by L-arginine.