大孔树脂纯化薏苡多糖的研究

Purification of polysaccharide from Coix lacryma-jobi L.by macroporous adsorption resin

为提高薏苡多糖纯度,以吸附量(率)与解吸率为评价指标,从6种大孔吸附树脂中筛选适宜的树脂,并采用静动态吸附-解吸方法优化工艺。结果表明:AB-8树脂纯化薏苡多糖效果更佳;其最适条件为:上柱液的质量浓度为0.68mg/mL,上柱液的体积为4BV,乙醇体积分数为60%,洗脱液体积为4BV。FT-IR图谱表明,薏苡多糖存在分子内和分子间的氢键,分子中存在C-H伸缩振动和-OH变形振动,存在呋喃糖苷吸收峰,是典型的多糖类化合物。

In order to improve the purity of polysaccharide,6 kinds of macroporous resin were chosen to carry out static and dynamic adsorption-desorption experiments.With the indexes of adsorption capacity and desorption rate,the suitable macroporous resin was selected and process parameters were confirmed.The results showed that AB-8 resin had the best performance of adsorption and desorption effects for purification of polysaccharide,the optimum technology parameters were sample concentration of 0.68mg/mL,sample volume of 4BV,60% ethanol for elution,elution volume of 4BV.The FT-IR spectral analysis showed that there were intramolecular hydrogen bond and intermolecular hydrogen bond.In addition,the existences of stretching vibration of the carbon-hydrogen and deformation vibration of hydroxyl group and characteristic absorption of ribofuranoside were also demonstrated.