两种海胆矿质元素的ICP-MS法测定分析

Determination of mineral elements in two kinds of sea urchin by ICP-MS method

利用电感耦合等离子体质谱法(ICP-MS)测定马粪海胆和紫海胆中K、Ca、Mg、Fe、V、Cr、Mn、Al、Co、Ni、Cu、Zn、As、Se、Mo、Cd、Hg、Pb共18种元素,并以内标元素补偿基体效应,选择适当的待测元素同位素克服质谱干扰,优化实验条件。用HNO3+HClO4(4:1)混酸作为消化液处理样品,各元素在实验范围内线性良好,相关系数均大于0.9984,加标回收率93.11%～116.91%,相对标准偏差(RSD)小于5%。结果表明:马粪海胆和紫海胆中K、Mg、Ca含量较高,且均含有Zn、Fe、Cu、V、Mn、Se等对人体非常有益的微量元素,为海胆的科学合理利用提供理论依据。

Eighteen kinds of mineral elements in sea urchin,Hemicentrotus pulcherrimus and Anthocidaris crassispina,were detected and analyzed by inductively coupled plasma mass spectrometry（ICP-MS）.6Li,45Sc,72Ge,89Y,115In,159Tb,209Bi were selected as the internal standard elements.The matrix effect was compensated and the MS interference was corrected by selecting the suitable isotope of the elements.The experimental conditions were optimized.The samples were digested with HNO3＋HClO4（4：1）.All the detected elements showed a good linear relationship within the working curves,the correlation coefficients were all above 0.9984.The precisions and recoveries were good,the range of recoveries（n=3） was 93.11%～116.91% and the RSDs were all lower than 5%.The results indicated that there were abundant nutritional elements for people in the two kinds of sea urichin.They were all rich in the elements K,Mg,Ca and some trace elements which are beneficial to humans such as Zn,Fe,Cu,V,Mn,Se.This study will provide some useful evidence for the effective exploitation of sea urichin.