摘要
目的:研究蟾酥注射液对人耐阿霉素(ADM)的急性髓性白血病细胞HL60/ADM多药耐药(MDR)的逆转作用。方法:采用MTT法分别考察蟾酥注射液对HL60/ADM细胞增殖能力和对ADM敏感性的变化,并采用高效液相色谱法考察HL60/ADM细胞对ADM的摄取能力。结果:蟾酥注射液能抑制细胞增殖;非细胞毒剂量蟾酥注射液预作用后,ADM对HL60/ADM细胞的半数抑制浓度(IC50)显著降低(P<0.01),HL60/ADM细胞对ADM摄取能力显著增加(P<0.01)。结论:蟾酥注射液可部分逆转HL60/ADM细胞对ADM的耐药性。
OBJECTIVE：To investigate the effects of Chansu injection on reversal of multi-drug resistance（MDR） of HL60/ADM cell.METHODS：MTT assay was used to investigate the effect of Chansu injection on the proliferation and the adramycin（ADM） sensitivity of HL60/ADM cell.HPLC method was applied to determine the ADM uptake abilities of HL60/ADM cell line.RESULTS：The proliferation of HL60/ADM cell was significantly inhibited by Chansu injection.When pretreated with Chansu injection in noncytotoxic dose,the IC50 of ADM in HL60/ADM cell was significantly reduced（P0.01）,and ADM uptake abilities of it enhanced significantly（P0.01）.CONCLUSION：Chansu injection can partially reverse the MDR of HL60/ADM cell.
出处
《中国药房》
CAS
CSCD
2012年第7期587-590,共4页
China Pharmacy
